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PXD002559

PXD002559 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteome Dynamics Mediate Revival of Bacterial Spores
DescriptionBackground: Bacterial spores can remain dormant for decades, yet harbor the exceptional capacity to rapidly resume metabolic activity and recommence life. Although germinants and their corresponding receptors have been known for more than 30 years, the molecular events underlying this remarkable cellular transition from dormancy to full metabolic activity are only partially defined. Results: Here, we examined whether protein phospho-modifications occur during germination, the first step of exiting dormancy, thereby facilitating spore revival. Utilizing Bacillus subtilis as a model organism, we performed phosphoproteomic analysis to define the Ser/Thr/Tyr phosphoproteome of a reviving spore. The phosphoproteome was found to chiefly comprise newly identified phosphorylation sites located within proteins involved in basic biological functions, such as transcription, translation, carbon metabolism and spore-specific determinants. Quantitative comparison of dormant and germinating spore phosphoproteomes revealed phosphorylation dynamics, indicating that phospho-modifications could modulate protein activity during this cellular transition. Furthermore, by mutating select phosphorylation sites located within proteins representative of key biological processes, we established a functional connection between phosphorylation and the progression of spore revival. Conclusions: In this study we provide for the first time a phosphoproteomic view of a germinating bacterial spore. We show that the spore phosphoproteome is dynamic and present evidence that phosphorylation events play an integral role in facilitating spore revival.
HostingRepositoryPRIDE
AnnounceDate2015-08-27
AnnouncementXMLSubmission_2015-09-20_23:54:46.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKarsten Krug
SpeciesList scientific name: Bacillus subtilis; NCBI TaxID: 1423;
ModificationListphosphorylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-20 01:27:32ID requested
12015-08-27 01:44:43announced
22015-09-20 23:54:47announcedUpdated publication reference for PubMed record(s): 26381121.
Publication List
Rosenberg A, Soufi B, Ravikumar V, Soares NC, Krug K, Smith Y, Macek B, Ben-Yehuda S, Phosphoproteome dynamics mediate revival of bacterial spores. BMC Biol, 13():76(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Bacillus subtilis
Spore
Phosphoproteome
Germination
Contact List
Boris Macek
contact affiliationProteome Center Tuebingen
contact emailboris.macek@uni-tuebingen.de
lab head
Karsten Krug
contact affiliationProteome Center
contact emailkarsten.krug@uni-tuebingen.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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