PXD002557

PXD002557 is an original dataset announced via ProteomeXchange.

Title	Akt2 partners L6 myoblasts
Description	Insulin resistance and Type 2 diabetes are marked by an aberrant response in the insulin signaling network. The phosphoinositide-dependent serine/threonine kinase, Akt2, plays a key role in insulin signaling and glucose uptake, most notably within skeletal muscle. Protein-protein interaction regulates the functional consequence of Akt2 and in turn, Akt2’s role in glucose uptake. However, only few insulin-responsive Akt2 interaction partners have been identified in skeletal muscle cells. In the present work, rat L6 myoblasts, a widely used insulin sensitive skeletal muscle cell line, were used to examine endogenous, insulin-stimulated Akt2 protein interaction partners. Akt2 co-immunoprecipitation was coupled with 1D-SDS-PAGE and fractions were analyzed by HPLC-ESI-MS/MS to reveal Akt2 protein-protein interactions. The pull-down assay displayed specificity for the Akt2 isoform; Akt1 and Akt3 unique peptides were not detected. A total of 330 proteins were significantly enriched as bonafide Akt2 protein interaction partners. Among them, 226 were novel for any cell types. Furthermore, 59 were detected with a significantly increased (57) or decreased (2) association with Akt2 following insulin administration (n=4; p<0.05). Multiple pathways were identified for the novel Akt2 interaction partners, such as the EIF2 and ubiquitination pathways. These data suggest that multiple new endogenous proteins may associate with Akt2 under basal as well as insulin-stimulated conditions, providing further insight into the insulin signaling network.
HostingRepository	PRIDE
AnnounceDate	2015-09-30
AnnouncementXML	Submission_2015-10-20_03:29:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Caruso
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-20 01:13:03	ID requested
1	2015-09-30 01:59:10	announced
⏵ 2	2015-10-20 03:29:01	announced	Updated publication reference for PubMed record(s): 26465754.

Caruso M, Zhang X, Ma D, Yang Z, Qi Y, Yi Z, Novel Endogenous, Insulin-Stimulated Akt2 Protein Interaction Partners in L6 Myoblasts. PLoS One, 10(10):e0140255(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Akt2, ROCK2, HPLC-ESI-MS/MS

Zhengping Yi
contact affiliation	Department of Phamaceutical Sciences Wayne State University
contact email	zhengping.yi@wayne.edu
lab head
Michael Caruso
contact affiliation	Wayne State University
contact email	ex3847@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002557
     1. Label: PRIDE project
     2. Name: Akt2 partners L6 myoblasts