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PXD002544

PXD002544 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology
DescriptionPlasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement.
HostingRepositoryPRIDE
AnnounceDate2015-08-10
AnnouncementXMLSubmission_2015-08-10_02:58:57.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJuliane Merl-Pham
SpeciesList scientific name: Populus canescens (Grey poplar) (Populus tremula x Populus alba); NCBI TaxID: 80863;
ModificationListdeamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-15 06:37:27ID requested
12015-08-10 02:58:59announced
Publication List
Bi Z, Merl-Pham J, Uehlein N, Zimmer I, M, ü, hlhans S, Aichler M, Walch AK, Kaldenhoff R, Palme K, Schnitzler JP, Block K, RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology. J Proteomics, 128():321-32(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: aquaporin
PIP, plasma membrane intrinsic protein
Populus × canescens
plasma membrane proteomics
photosynthesis
stomatal movement
Contact List
Jörg-Peter Schnitzler
contact affiliationResearch Unit Environmental Simulation, Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, Ingolstädter Landstr.1 85764 Neuherberg, Germany
contact emailjp.schnitzler@helmholtz-muenchen.de
lab head
Juliane Merl-Pham
contact affiliationResearch Unit Protein Science, Helmholtz Center Munich
contact emailjuliane.merl@helmholtz-muenchen.de
dataset submitter
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