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PXD002539

PXD002539 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic study of fruit exocarp tissue in four cultivars of Vitis Vinifera
DescriptionThe aim of this work was to study the metabolism of grape berry skin, a tissue that has a protective role against damage by physical injuries and pathogen attacks. This role requires a metabolism able to sustain biosynthetic activities such as those relating to secondary compounds (i.e. flavonoids). In order to draw the attention on these biochemical processes, a proteomic and metabolomic comparative analysis was performed among Riesling Italico, Pinot Gris, Pinot Noir and Croatina cultivars, which are known to accumulate anthocyanins to a different extent. The application of multivariate statistics on the dataset pointed out that the cultivars were distinguishable from each other and the order in which they were grouped mainly reflected their relative anthocyanin contents. Sorting the spots according to their significance selected proteins were characterized by LC-ESI-MS/MS. Considering the functional distribution, the identified proteins were involved in many physiological processes such as stress, defense, carbon metabolism, energy conversion and secondary metabolism. The trends of some metabolites were related to those of the protein data. Taken together, the results permitted to highlight the relationships between the secondary compound pathways and the main metabolism (e.g. glycolysis and TCA cycle). Moreover, the trend of accumulation of many proteins involved in stress responses, reinforced the idea that they could play a role in the cultivar specific developmental plan.
HostingRepositoryPRIDE
AnnounceDate2015-07-27
AnnouncementXMLSubmission_2015-08-31_02:39:18.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLuca Espen
SpeciesList scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760;
ModificationListiodoacetamide derivatized residue
InstrumentLCQ Classic
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-14 06:47:36ID requested
12015-07-27 03:22:33announced
22015-08-31 02:39:19announcedUpdated publication reference for PubMed record(s): 26300900.
Publication List
Negri AS, Prinsi B, Failla O, Scienza A, Espen L, Proteomic and metabolic traits of grape exocarp to explain different anthocyanin concentrations of the cultivars. Front Plant Sci, 6():603(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Grape berry, Vitis vinifera, 2-DE
Contact List
Luca Espen
contact affiliationDipartimento di Scienze Agrarie e Ambientali, Produzione, Territorio, Agroenergia (DISAA), Università degli Studi di Milano, Milan - ITALY
contact emailluca.espen@unimi.it
lab head
Luca Espen
contact affiliationUniversità degli Studi di Milano
contact emailluca.espen@unimi.it
dataset submitter
Full Dataset Link List
Dataset FTP location
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