PXD002538

PXD002538 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Network Analysis Reveals Molecular Mechanisms of Myometrial Stretch Response
Description	Mechanical stress is a potent regulator of cell growth, contractility and gene regulation. Abnormal uterine distension during pregnancy increases the risk of preterm birth and likely activates crosstalk between multiple signaling networks with protein phosphorylation playing a critical role. Telomerized human uterine smooth muscle cells were exposed to 18% biaxial stretch for 5 min and the phosphoproteome was probed by mass spectrometry. We observed specific phospho-activation of mitogen activated protein kinase at threonine 183 and tyrosine 185, myosin regulatory light chain 9 at threonine 19, and heat shock protein 27 at serine 82. Our analysis revealed protein phosphorylation changes in signaling pathways related to actin cytoskeleton remodeling, activation of the focal adhesion kinase pathway, smooth muscle contraction and mechanistic target of rapamycin activation. These data point to potential mechanistic links between stretch-induced phosphorylation and development of the contractile phenotype in myometrial cells.
HostingRepository	PRIDE
AnnounceDate	2019-04-10
AnnouncementXML	Submission_2019-04-10_06:16:41.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002538
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Craig Ulrich
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; TMT6plex; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-14 02:07:23	ID requested
⏵ 1	2019-04-10 06:16:42	announced

Copley Salem C, Ulrich C, Quilici D, Schlauch K, Buxton ILO, Burkin H, Mechanical strain induced phospho-proteomic signaling in uterine smooth muscle cells. J Biomech, 73():99-107(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Phosphoproteomic, Human, uterus, myometrium, stretch, distension, cell culture

Heather Burkin
contact affiliation	Associate Professor, University of Nevada, Reno department of Pharmacology
contact email	hburkin@medicine.nevada.edu
lab head
Craig Ulrich
contact affiliation	Pharmacology
contact email	culrich@medicine.nevada.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002538
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Network Analysis Reveals Molecular Mechanisms of Myometrial Stretch Response