PXD002534

PXD002534 is an original dataset announced via ProteomeXchange.

Title	A method to characterize antibody selectivity immunoprecipitation, part 2
Description	Antibodies are used in multiple cell biology applications but there are methods to assess antibody quality, riskingdata integrity and repr developed a standard operating procedure using HEK293 cells to score and antibodies for suitability in immunoprecipitation. This method, validated in in fourindependent laboratories, was developed using 1124 novel recomb for152 chromatin-related human proteins. We used mass spectrometry abundanceof all the proteinsin eachimmunoprecipitateand compared spectral abundance factors from the target antigen with those of all Antibodies for which the targetantigen or a member of its known protein most abundant protein were classified as “IP gold standard”. This m quantitative outputs that can be stored and archived in public databases, step towards a platform for community benchmarking of antibody quality.
HostingRepository	PRIDE
AnnounceDate	2015-07-16
AnnouncementXML	Submission_2015-07-15_18:58:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongbo Guo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-13 09:08:10	ID requested
⏵ 1	2015-07-15 18:58:20	announced

Marcon E, Jain H, Bhattacharya A, Guo H, Phanse S, Pu S, Byram G, Collins BC, Dowdell E, Fenner M, Guo X, Hutchinson A, Kennedy JJ, Krastins B, Larsen B, Lin ZY, Lopez MF, Loppnau P, Miersch S, Nguyen T, Olsen JB, Paduch M, Ravichandran M, Seitova A, Vadali G, Vogelsang MS, Whiteaker JR, Zhong G, Zhong N, Zhao L, Aebersold R, Arrowsmith CH, Emili A, Frappier L, Gingras AC, Gstaiger M, Paulovich AG, Koide S, Kossiakoff AA, Sidhu SS, Wodak SJ, Gr, ä, slund S, Greenblatt JF, Edwards AM, Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation. Nat Methods, 12(8):725-31(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: antibody selectivity and specificity, immunoprecipitation, mass spectrometry

Jack Greenblatt
contact affiliation	University of Toronto
contact email	jack.greenblatt@utoronto.ca
lab head
Hongbo Guo
contact affiliation	University of Toronto
contact email	hongboguo88@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD002534

PRIDE project URI

• PRIDE
  1. PXD002534
     1. Label: PRIDE project
     2. Name: A method to characterize antibody selectivity immunoprecipitation, part 2