PXD002509

PXD002509 is an original dataset announced via ProteomeXchange.

Title	Outer membrane vesicle proteome of Aggregatibacter actinomycetemcomitans serotype e strain 173
Description	Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis, and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells, and to act as triggers of innate immunity upon carriage of NOD1- and NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding of the pathogenicity-associated functions that A. actinomycetemcomitans exports via OMVs, we have used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize the OMV-associated proteome of a rough-colony type clinical isolate, strain 173 (serotype e). This yielded identification of 151 proteins overlapping in three out of four independent experiments, using density gradient-purified OMVs. Further to confirming the vesicle-associated release of LtxA, and of several proteins earlier demonstrated to act as immunoreactive antigens in the human host, we identified numerous additional putative virulence-related proteins in the A. actinomycetemcomitans OMV proteome, including proteins involved in immune evasion, drug targeting, and iron/nutrient acquisition. In summary our findings are consistent with an OMV-associated proteome that exhibits several offensive and defensive functions, and they provide a comprehensive basis to further disclose roles of A. actinomycetemcomitans OMVs in periodontal and systemic disease.
HostingRepository	PRIDE
AnnounceDate	2015-09-21
AnnouncementXML	Submission_2015-09-21_04:12:38.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002509
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Kieselbach
SpeciesList	scientific name: Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans); NCBI TaxID: 714;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-09 02:29:05	ID requested
⏵ 1	2015-09-21 04:12:39	announced

Kieselbach T, Zijnge V, Granstr, ö, m E, Oscarsson J, Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles. PLoS One, 10(9):e0138591(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Aggregatibacter actinomycetemcomitans, gram negative, outer membrane vesicles, LC-MS/MS

Thomas Kieselbach
contact affiliation	Umeå University, Department of Chemistry, 90187 Umeå, Sweden
contact email	thomas.kieselbach@chem.umu.se
lab head
Thomas Kieselbach
contact affiliation	Biochemistry
contact email	thomas.kieselbach@chem.umu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002509
     1. Label: PRIDE project
     2. Name: Outer membrane vesicle proteome of Aggregatibacter actinomycetemcomitans serotype e strain 173