PXD002501

PXD002501 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of Mycoplasma pneumoniae clinical isolates
Description	The human respiratory tract pathogen M. pneumoniae is one of the best characterized minimal bacterium. Until now, two main groups of clinical isolates of this bacterium have been described (types 1 and 2), differing in the sequence of the P1 adhesin gene. Here, we have sequenced the genomes of 23 clinical isolates of M. pneumoniae. Studying SNPs, non-synonymous mutations, indels and genome rearrangements of these 23 strains and 4 previously sequenced ones, has revealed new subclasses in the two main groups, some of them being associated with the country of isolation. Integrative analysis of in vitro gene essentiality and mutation rates enabled the identification of several putative virulence factors and antigenic proteins; revealing recombination machinery, glycerol metabolism and peroxide production as key factors in the genetics and physiology of these pathogenic strains. Additionally, the transcriptomes and proteomes of two representative strains, one from each of the two main groups, have been characterized to evaluate the impact of mutations on RNA and proteins levels. This study has revealed that type 2 strains show high expression levels of CARDs toxin, a protein recently shown to be one of the major factors of inflammation. Thus, we propose that type 2 strains are likely to be more virulent than type 1 strains.
HostingRepository	PRIDE
AnnounceDate	2016-07-05
AnnouncementXML	Submission_2016-07-05_03:29:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Javier Delgado
SpeciesList	scientific name: Mycoplasma pneumoniae M129-B7; NCBI TaxID: 1238993;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-08 03:31:53	ID requested
⏵ 1	2016-07-05 03:29:33	announced

Lluch-Senar M, Cozzuto L, Cano J, Delgado J, Ll, ó, rens-Rico V, Pereyre S, Bebear C, Serrano L, Comparative "-omics" in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence Factors. PLoS One, 10(9):e0137354(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: mycoplasma pnemoniae, proteomics

Luis Serrano
contact affiliation	- EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), Dr. Aiguader 88, 08003 Barcelona, Spain - Universitat Pompeu Fabra (UPF), Dr. Aiguader 88, 08003 Barcelona, Spain. - Institució Catalana de Recerca i Estudis Avançats (ICREA), Pg. Lluis Companys 23, 08010 Barcelona, Spain
contact email	luis.serrano@crg.eu
lab head
Javier Delgado
contact affiliation	CRG
contact email	javier.delgado@crg.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD002501

PRIDE project URI

• PRIDE
  1. PXD002501
     1. Label: PRIDE project
     2. Name: Comparative proteomics of Mycoplasma pneumoniae clinical isolates