PXD002498

PXD002498 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics in osteoblasts-released matrix vesicles
Description	The use of glycosaminoglycan-coated implants are a promising therapeutic approach in bone research since they mimic the native bone environment and facilitate the skeletonegenesis. Previous studies showed that sulfated glycosaminoglycans induce the osteoblastic phenotype, but the overall effect on the cellular proteome were only minor. Therefore we focussed on the osteoblast-released matric vesicles (MV) that are known to hold a keyrole in the initiation of the mineralization of the extracellular matrix. Indeed, we found a strong impact of sulfated glycosaminoglycans on the activity and composition of MV. In addition, to find out if the altered MV composition is a reflection of an altered cellular proteome we compared the GAG-induced changes in both proteome.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_00:48:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes R. Schmidt
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-08 01:39:59	ID requested
1	2015-11-30 02:07:06	announced
2	2017-06-21 00:31:06	announced	Updated project metadata.
⏵ 3	2017-10-24 00:48:06	announced	Updated project metadata.

Schmidt JR, Kliemt S, Preissler C, Moeller S, von Bergen M, Hempel U, Kalkhof S, Osteoblast-released Matrix Vesicles, Regulation of Activity and Composition by Sulfated and Non-sulfated Glycosaminoglycans. Mol Cell Proteomics, 15(2):558-72(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, osteoblasts, matrix vesicles, exosome, LC-MSMS, SILAC, glycosaminoglycans

Martin von Bergen
contact affiliation	Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Johannes R. Schmidt
contact affiliation	Helmholtz Centre for Environmental Research - UFZ
contact email	johannes.schmidt@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002498

PRIDE project URI

• PRIDE
  1. PXD002498
     1. Label: PRIDE project
     2. Name: Quantitative proteomics in osteoblasts-released matrix vesicles