PXD002494

PXD002494 is an original dataset announced via ProteomeXchange.

Title	Identification of GLI-associated proteins by mass spectrometry
Description	The Hedgehog signaling pathway is essential for the maintenance and response of several types of stem cells. To study the transcriptional response of stem cells to HH signaling, we searched for proteins binding to GLI proteins, the transcriptional effectors of the HH pathway in mouse embryonic stem (ES) cells. We purified GLI protein complex from an ES cell line that contained a tamoxifen-inducible 3XFLAG-tagged GLI3 repressor allele by anti-FLAG immunoprecipitation and several novel GLI co-factors were identified in the complex by subsequent mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2016-02-04
AnnouncementXML	Submission_2016-02-04_00:02:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Person
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-07 01:51:47	ID requested
⏵ 1	2016-02-04 00:02:52	announced

Li Q, Lex RK, Chung H, Giovanetti SM, Ji Z, Ji H, Person MD, Kim J, Vokes SA, The Pluripotency Factor NANOG Binds to GLI Proteins and Represses Hedgehog-mediated Transcription. J Biol Chem, 291(13):7171-82(2016) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Embryonic Stem Cells, GLI, Hedgehog signaling, NANOG

Steven A. Vokes
contact affiliation	The University of Texas at Austin Department of Molecular Biosciences Institute for Cellular and Molecular Biology
contact email	svokes@austin.utexas.edu
lab head
Maria Person
contact affiliation	University of Texas at Austin
contact email	mperson@austin.utexas.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002494

PRIDE project URI

• PRIDE
  1. PXD002494
     1. Label: PRIDE project
     2. Name: Identification of GLI-associated proteins by mass spectrometry