PXD002491

PXD002491 is an original dataset announced via ProteomeXchange.

Title	The Hepatocyte Proteome in Organotypic Rat Liver Models and the Influence of the Local Microenvironment
Description	Liver models that closely mimic the in vivo microenvironment are central for understanding liver functions, capabilities, and intercellular communication processes. Whereas hepatocyte monolayers de-differentiate after only a few days in culture, constructs consisting of hepatocytes and non-parenchymal cells (NPC) separated by a polyelectrolyte multilayer (PEM) provide maintenance of hepatic phenotypes. To better understand the mechanisms and processes that underlie organotypic liver model function, hepatocyte protein abundances in the presence and absence of liver sinusoidal endothelial cells (LSECs) were compared to hepatocyte monolayers. The presence of the PEM led to increases in proteins associated with hepatocyte lipid metabolism, with mitochondrial-based β-oxidation and peroxisomal proteins found in higher abundance. It was also demonstrated that the presence of LSECs modulates levels of carboxylesterases and other phase I and phase II enzymes. These results are discussed in relation to intercellular signaling and hepatocyte function.
HostingRepository	PRIDE
AnnounceDate	2017-07-06
AnnouncementXML	Submission_2017-07-06_03:04:16.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002491
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lucas Vu
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue: 57.02146; monohydroxylated residue: 15.99491; deamidated residue: 0.984
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 08:45:03	ID requested
⏵ 1	2017-07-06 03:04:17	announced

Vu LT, Orbach SM, Ray WK, Cassin ME, Rajagopalan P, Helm RF, The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment. Proteome Sci, 15():12(2016) [pubmed]

curator keyword: Biological

submitter keyword: Hepatocyte, Liver, in vitro, organotypic

Richard F. Helm
contact affiliation	Department of Biochemistry, Mass Spectrometry Incubator, Virginia Polytechnic Institute and State University
contact email	helmrf@vt.edu
lab head
Lucas Vu
contact affiliation	Virginia Tech
contact email	ltvu2@vt.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002491
     1. Label: PRIDE project
     2. Name: The Hepatocyte Proteome in Organotypic Rat Liver Models and the Influence of the Local Microenvironment