PXD002489

PXD002489 is an original dataset announced via ProteomeXchange.

Title	Cupiennius salei Hyal LC-MSMS
Description	Hyaluronidases are important venom components by acting as spreading factor of neurotoxins. In several studies this spreading effect was tested on vertebrate tissue. However, data about the spreading activity on invertebrates, which are the main prey organisms of spiders, are lacking. In this study, a hyaluronidase-like enzyme was isolated from the venom of the spider Cupiennius salei. The amino acid sequence of the enzyme was solved by cDNA analysis of the venom gland transcriptome and confirmed by protein analytics. Two complex N-linked glycans, which akin honey bee hyaluronidase glycosylations, were identified by tandem mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2015-12-10
AnnouncementXML	Submission_2015-12-10_09:16:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Trachsel
SpeciesList	scientific name: Cupiennius salei (American wandering spider); NCBI TaxID: 6928;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 08:12:40	ID requested
⏵ 1	2015-12-10 09:16:56	announced

Biner O, Trachsel C, Moser A, Kopp L, Langenegger N, K, ä, mpfer U, von Ballmoos C, Nentwig W, Sch, ü, rch S, Schaller J, Kuhn-Nentwig L, Isolation, N-glycosylations and Function of a Hyaluronidase-Like Enzyme from the Venom of the Spider Cupiennius salei. PLoS One, 10(12):e0143963(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Cupiennius salei, spider, venom, hyaluronidase

Lucia Kuhn-Nentwig
contact affiliation	Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland
contact email	lucia.kuhn@iee.unibe.ch
lab head
Christian Trachsel
contact affiliation	Functional Genomics Center Zurich
contact email	christian.trachsel@fgcz.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/12/PXD002489

PRIDE project URI

• PRIDE
  1. PXD002489
     1. Label: PRIDE project
     2. Name: Cupiennius salei Hyal LC-MSMS