PXD002484

PXD002484 is an original dataset announced via ProteomeXchange.

Title	2-DE proteomics analysis of drought treated seedlings of Quercus ilex
Description	Applying a gel-based proteomic approach, the dynamic changes in root proteins of drought treated Quercus ilex subsp. Ballota [Desf.] Samp. seedlings were followed. Water stress was applied on 20 day-old holm oak plantlets by water limitation for a period of 10 and 20 days, each followed by 10 days of recovery. Root proteins were extracted using trichloroacetate/acetone/phenol protocol and subjected to two-dimensional electrophoresis. Coomassie colloidal stained gel images were analysed and spot intensity data subjected to multivariate statistical analysis. Selected consistent spots in the three biological replicas, presenting significant changes under stress, were subjected to MALDI-TOF mass spectrometry (peptide mass fingerprinting and MS/MS). For protein identification, combined search was performed with MASCOT search engine over NCBInr Viridiplantae and Uniprot databases. Taxonomy Holm oak (Quercus ilex subsp. Ballota [Desf.] Samp.). Dentro de Q. ilex hay dos subespecies, ilex ilex e ilex Ballota.
HostingRepository	PRIDE
AnnounceDate	2015-09-09
AnnouncementXML	Submission_2015-09-09_06:34:14.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002484
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	J. Alberto Medina-Aunon
SpeciesList	scientific name: Quercus ilex (Holly oak); NCBI TaxID: 58334;
ModificationList	monohydroxylated residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 02:30:46	ID requested
⏵ 1	2015-09-09 06:34:15	announced

Simova-Stoilova LP, Romero-Rodr, í, guez MC, S, á, nchez-Lucas R, Navarro-Cerrillo RM, Medina-Aunon JA, Jorr, í, n-Novo JV, 2-DE proteomics analysis of drought treated seedlings of Quercus ilex supports a root active strategy for metabolic adaptation in response to water shortage. Front Plant Sci, 6():627(2015) [pubmed]

curator keyword: Biological

submitter keyword: 2D-E, MALDI, holm oak, roots, drought, recovery, proteomics

Jesus Jorrin Novo
contact affiliation	Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Cordoba, Agrifood Campus of International Excellence (ceiA3), 14071 Cordoba, Spain
contact email	bf1jonoj@uco.es
lab head
J. Alberto Medina-Aunon
contact affiliation	Proteomics - Bioinformatics
contact email	jamedina@proteored.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD002484

PRIDE project URI

• PRIDE
  1. PXD002484
     1. Label: PRIDE project
     2. Name: 2-DE proteomics analysis of drought treated seedlings of Quercus ilex