PXD002483

PXD002483 is an original dataset announced via ProteomeXchange.

Title	EV71 proteomics
Description	Enterovirus 71 (EV71), a member of Picornaviridae, causes severe neurological and systemic illness in children. To better understand the virus-host cell interactions, we performed a triple-SILAC-based quantitative proteomics study monitoring host cell proteome changes after EV71 infection. Based on the quantitative data for more than 4100 proteins, ~17% of the proteins were found as significantly changed (p<0.01) at either 8 or 20 hours post infection (h.p.i.). Five biological processes and seven protein classes showed significant differences. Functional screening of 9 regulated proteins discovered the regulatory role of CHCH2, a mitochondrial protein known as a transcriptional activator for cytochrome c oxidase (COX), in EV71 replication. Further studies showed that CHCH2 served as a negative regulator of innate immune responses.
HostingRepository	PRIDE
AnnounceDate	2015-08-31
AnnouncementXML	Submission_2015-08-31_02:22:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoyu Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 02:22:51	ID requested
⏵ 1	2015-08-31 02:22:47	announced

Li HY, Zhang LK, Zhu XJ, Shang J, Chen X, Zhu Y, Guo L, Analysis of EV71 infection progression using triple-SILAC-based proteomics approach. Proteomics, 15(21):3629-43(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Enterovirus 71

TripleTOF 5600

Triple SILAC

proteomics;

Lin Guo
contact affiliation	College of Life Sciences, Wuhan University, Wuhan 430072, China
contact email	guol@whu.edu.cn
lab head
Haoyu Li
contact affiliation	Wuhan University
contact email	haoyuli@whu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002483

PRIDE project URI

• PRIDE
  1. PXD002483
     1. Label: PRIDE project
     2. Name: EV71 proteomics