PXD002481

PXD002481 is an original dataset announced via ProteomeXchange.

Title	Pig embryonic muscle iTRAQ analysis
Description	Here, we performed the first iTRAQ-based proteomic analysis of early embryonic longissimus dorsi muscle from Landrance (LR) and miniature pig (Wuzhishan WZS) ranging from 21 to 42 days post coitus (dpc). 4431 proteins were identified. In both WZS and LR, the largest amount of differentially expressed proteins (DEPs) were found between 28 and 35 dpc. 252 breed-DEPs were selected by GO analysis, including 8 myofibrillar proteins. Only MYHCⅠ/IIA mRNA were detected due to early embryonic stages, and significantly higher expression of them were found in WZS during these 4 stages. MYHCⅠ was first found in WZS at 28 dpc and expressed in both breeds at later stages, while MYHCII protein was not detected until 35 dpc in both breeds. Thus, 33 myogenic breed-DEPs selected from last two stages were analyzed by STRING, which showed that some myofibrillar proteins (MYH1, TPM4, MYH10, et.al) and functional proteins (CSRP2, CASQ2, OTC, et.al), together with candidate myogenic proteins (H3F3A, HDGFRP2, et.al), probably participate in the regulatory network of myofiber formation. Thus, our study provides new data on myofiber type development during early embryo stages, which contributes to the improved understanding in the regulation mechanism of early myogenesis in agricultural animals.
HostingRepository	PRIDE
AnnounceDate	2016-02-26
AnnouncementXML	Submission_2016-02-26_01:44:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xumeng Zhang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 01:46:38	ID requested
⏵ 1	2016-02-26 01:44:29	announced

Zhang X, Chen Y, Pan J, Liu X, Chen H, Zhou X, Yuan Z, Wang X, Mo D, iTRAQ-based quantitative proteomic analysis reveals the distinct early embryo myofiber type characteristics involved in landrace and miniature pig. BMC Genomics, 17():137(2016) [pubmed]

curator keyword: Biological

submitter keyword: Embyro myogenesis/iTRAQ/Muscle proteome/Pig/Quantitative proteomics

Xumeng Zhang
contact affiliation	State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510006, Guangdong, China,
contact email	zhangxum@mail2.sysu.edu.cn
lab head
Xumeng Zhang
contact affiliation	Sun Yat-sen University
contact email	zhangxum@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002481

PRIDE project URI

• PRIDE
  1. PXD002481
     1. Label: PRIDE project
     2. Name: Pig embryonic muscle iTRAQ analysis