PXD002480

PXD002480 is an original dataset announced via ProteomeXchange.

Title	Facile Discovery of Cell-surface Protein Targets of Cancer Cell Aptamers
Description	Cancer biomarker discovery constitutes a frontier in cancer research. In recent years, cell-binding aptamers have become useful molecular probes for biomarker discovery. However, there are few successful examples, and the critical barrier resides in the identification of the cell-surface protein targets for the aptamers, where only a limited number of aptamer targets have been identified so far. Herein, we developed a universal SILAC-based quantitative proteomic method for target discovery of cell-binding aptamers. The method allowed for distinguishing specific aptamer-binding proteins from non-specific proteins based on abundance ratios of proteins bound to aptamer-carrying bait and control bait. In addition, we employed fluorescently labeled aptamers for monitoring and optimizing the binding conditions. We were able to identify and validate selectin L and integrin 4 as the protein targets for two previously reported aptamers, Sgc-3b and Sgc-4e, respectively. This strategy should be generally applicable for the discovery of protein targets for other cell-binding aptamers, which will promote the applications of these aptamers.
HostingRepository	PRIDE
AnnounceDate	2022-02-28
AnnouncementXML	Submission_2022-02-28_02:21:41.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tao Bing
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 01:33:38	ID requested
⏵ 1	2022-02-28 02:21:41	announced

Bing T, Shangguan D, Wang Y, Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers. Mol Cell Proteomics, 14(10):2692-700(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Affinity proteomics, Aptamers, Cancer biomarker(s), Quantification, SILAC.

Yinsheng Wang
contact affiliation	Department of Chemistry, University of California, Riverside, CA92521-0403, USA.
contact email	Yinsheng.Wang@ucr.edu
lab head
Tao Bing
contact affiliation	Institute of Chemistry, Chinese Academy of Sciences
contact email	bingtao@iccas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002480
     1. Label: PRIDE project
     2. Name: Facile Discovery of Cell-surface Protein Targets of Cancer Cell Aptamers