PXD002479

PXD002479 is an original dataset announced via ProteomeXchange.

Title	Overexpression of catalase delays age-related cardiac disease by preventing oxidative cysteine modifications
Description	Reactive protein cysteine thiolates are instrumental in redox regulation. Aging associated to oxidative stress, can impair redox signaling by damaging essential cysteine thiolates. Our initial study found that cardiac-specific overexpression of catalase, can protect from oxidative stress and delay cardiac aging in mice. The Project aimed to investigate differential reversible cysteine thiol oxidation in cardiac proteome of wild type and Cat transgenic (Tg) mice, using “Tandem Mass Tag” (TMT) labeling strategy and mass spectrometry. Our results show globally decreased cysteine thiol occupancy in cardiac proteins in Cat Tg mice. The majority of proteins with differentially modified thiols may be involved in pathways of aging and cardiac disease including cellular stress response, proteostasis and apoptosis. Overexpressed catalase may modulate these protein cysteine thiol oxidations resulting in delayed cardiac aging and related pathologies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:36.389.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Bachschmid
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-06 01:31:00	ID requested
1	2018-10-24 10:07:17	announced
⏵ 2	2024-10-22 04:05:42	announced	2024-10-22: Updated project metadata.

Yao C, Behring JB, Shao D, Sverdlov AL, Whelan SA, Elezaby A, Yin X, Siwik DA, Seta F, Costello CE, Cohen RA, Matsui R, Colucci WS, McComb ME, Bachschmid MM, Overexpression of Catalase Diminishes Oxidative Cysteine Modifications of Cardiac Proteins. PLoS One, 10(12):e0144025(2015) [pubmed]

10.1371/journal.pone.0144025;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

curator keyword: Biological, Biomedical

submitter keyword: metabolism, proteomics,Reversible cysteine thiol oxidation, TMT, post-translational protein modifications, mass spectrometry, cardiac aging and disease

Richard A. Cohen
contact affiliation	Vascular Biology Section, Boston University School of Medicine, Boston, MA
contact email	racohen@bu.edu
lab head
Markus Bachschmid
contact affiliation	Quintara Biosciences
contact email	bachschmid.markus@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002479
     1. Label: PRIDE project
     2. Name: Overexpression of catalase delays age-related cardiac disease by preventing oxidative cysteine modifications