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PXD002471

PXD002471 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative histone mass spectrometry identifies elevated histone H3 lysine 27 trimethylation in melanoma
DescriptionNormal cell growth is characterized by a regulated epigenetic program that drives cellular activities such as gene transcription, DNA replication and DNA damage repair. Perturbation of this epigenetic program can lead to events such as mis-regulation of gene transcription and diseases such as cancer. To begin to understand the epigenetic program correlated to the development of melanoma, we performed a quantitative mass spectrometric analysis of histone posttranslational modifications mis-regulated in melanoma cell culture. Aggressive melanoma cells were found to have elevated histone H3 lysine 27 trimethylation (H3K27me3) as well as over-expressed methyltransferase EZH2 that adds the specific modification. The altered epigenetic program that led to elevated H3K27me3 in melanoma cell culture was found to directly silence transcription of the tumor suppressor gene RUNX3. The elevated level of H3K27me3 and silencing of RUNX3 transcription was also validated in advanced stage human melanoma tissues. The study presented underscores the utility of using high resolution mass spectrometry to identify mis-regulated epigenetic programs in diseases such as cancer, which could ultimately lead to the identification of biological markers for diagnostic and prognostic applications.
HostingRepositoryPRIDE
AnnounceDate2015-12-02
AnnouncementXMLSubmission_2015-12-02_04:08:43.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002471
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterStephanie Byrum
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListDimethyl; Phospho; Acetyl:2H(3); Trimethyl; Methyl; Acetyl; Carbamidomethyl; Methyl+Acetyl:2H(3)
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-03 01:39:56ID requested
12015-12-02 04:08:45announced
Publication List
Sengupta D, Byrum SD, Avaritt NL, Davis L, Shields B, Mahmoud F, Reynolds M, Orr LM, Mackintosh SG, Shalin SC, Tackett AJ, Quantitative Histone Mass Spectrometry Identifies Elevated Histone H3 Lysine 27 (Lys27) Trimethylation in Melanoma. Mol Cell Proteomics, 15(3):765-75(2016) [pubmed]
Keyword List
submitter keyword: Human, melanoma, histone H3 lysine 27 trimethylation, RUNX3, EZH2
Contact List
Stephanie Byrum
contact affiliationDepartment of Biochemistry and Molecular Biology University of Arkansas for Medical Sciences USA
contact emailsbyrum@uams.edu
lab head
Stephanie Byrum
contact affiliationUAMS
contact emailsbyrum@uams.edu
dataset submitter
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Dataset FTP location
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