PXD002470

PXD002470 is an original dataset announced via ProteomeXchange.

Title	iTRAQ Manatee .
Description	In 2013, two large-scale Florida manatee (Trichechus manatus latirostris) mortality episodes were reported on separate coasts of Florida. The east coast mortality episode was associated with an unknown etiology in the Indian River Lagoon (IRL). The west coast mortality episode was attributed to a persistent Karenia brevis algal bloom or ‘red tide’ centered in Southwest Florida. To investigate these two mortality episodes, proteomic experiments using two-dimensional difference gel electrophoresis (2D-DIGE) followed by protein identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were conducted, along with a separate gel-free analysis using isobaric tags for relative and absolute quantification (iTRAQ) LC-MS/MS. In comparison to the control group, manatees from the IRL, an area associated with an unknown mortality episode, displayed increased levels of several proteins in their serum samples. These increased proteins, which were identified in the iTRAQ experiment, included kininogen-1 isoform 1 (average ratio 1.38), protein AMBP (1.38), histidine-rich glycoprotein (1.34), properdin (1.30), and complement C4-A isoform 1 (1.25). In the red tide group, ceruloplasmin (2.32), pyruvate kinase isozymes M1/M2 isoform 3 (2.29), angiotensinogen (2.08), complement C4-A isoform 1 (1.83), and complement C3 (1.42) were increased. The proteins kininogen-1 isoform 1, histidine-rich glycoprotein, complement C4-A isoform 1, angiotensinogen, and complement C3 were also identified in increased levels in the 2D-DIGE experiment (Table 2b). These proteins are associated with acute-phase response, amyloid formation and accumulation, copper and iron homeostasis, the complement cascade pathway, and other important cellular functions. The increased level of complement C4 protein observed in both the red tide and unknown mortality episode groups was confirmed through the use of Western Blot.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_07:59:52.321.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002470
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebecca Lazensky
SpeciesList	scientific name: Trichechus manatus (Caribbean manatee) (West Indian manatee); NCBI TaxID: 9778;
ModificationList	Oxidation; iTRAQ8plex; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-02 01:41:39	ID requested
⏵ 1	2024-10-03 07:59:53	announced

10.6019/PXD002470;

10.1038/s41598-021-83687-y;

Lazensky R, Silva-Sanchez C, Kroll KJ, Chow M, Chen S, Tripp K, Walsh MT, Denslow ND, Investigating an increase in Florida manatee mortalities using a proteomic approach. Sci Rep, 11(1):4282(2021) [pubmed]

curator keyword: Biological

submitter keyword: iTRAQ Mass spectrometry,Manatee

Dr. Nancy Denslow
contact affiliation	Center for Environmental and Human Toxicology Department of Physiological Sciences & College of Medicine College of Veterinary Medicine University of Florida
contact email	ndenslow@ufl.edu
lab head
Rebecca Lazensky
contact affiliation	Student
contact email	beckylazensky@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD002470

PRIDE project URI

• PRIDE
  1. PXD002470
     1. Label: PRIDE project
     2. Name: iTRAQ Manatee