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PXD002467

PXD002467 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioration in Old Rats
DescriptionAging is associated with the decline of protein, cell, and organ function. Here, we use an integrated approach to characterize gene expression, bulk translation, and cell biology in the brains and livers of young and old rats. We identify 468 differences in protein abundance between young and old animals. The majority are a consequence of altered translation output, that is, the combined effect of changes in transcript abundance and translation efficiency. In addition, we identify 130 proteins whose overall abundance remains unchanged but whose sub-cellular localization, phosphorylation state, or splice-form varies. While some protein-level differences appear to be a generic property of the rats’ chronological age, the majority are specific to one organ. These may be a consequence of the organ’s physiology or the chronological age of the cells within the tissue. Taken together, our study provides an initial view of the proteome at the molecular, sub-cellular, and organ level in young and old rats. The corresponding dataset in Gene Expression Omnibus is GSE66715.
HostingRepositoryPRIDE
AnnounceDate2017-10-24
AnnouncementXMLSubmission_2017-10-24_05:54:07.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlessandro Ori
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListphosphorylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-07-02 00:48:47ID requested
12015-10-05 03:47:14announced
22016-05-03 01:18:39announcedUpdated publication reference for PubMed record(s): 27135913.
32016-05-03 01:42:08announcedUpdated project metadata.
42017-06-20 05:43:40announcedUpdated project metadata.
52017-10-24 05:54:08announcedUpdated project metadata.
Publication List
Ori A, Toyama BH, Harris MS, Bock T, Iskar M, Bork P, Ingolia NT, Hetzer MW, Beck M, Integrated Transcriptome and Proteome Analyses Reveal Organ-Specific Proteome Deterioration in Old Rats. Cell Syst, 1(3):224-37(2015) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: aging, brain, liver, rat
Contact List
Martin Beck
contact affiliationEuropean Molecular Biology Laboratory
contact emailmartin.beck@embl.de
lab head
Alessandro Ori
contact affiliationEMBL
contact emailaleori@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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