PXD002466

PXD002466 is an original dataset announced via ProteomeXchange.

Title	Wdr13-/0 compared to wild-type mice hippocampus and pre-frontal cortex iTRAQ proteomics
Description	Wdr13 has been implicated in memory and mental disorders, particularly in X-linked intellectual disability (XLID) in animal studies and in humans. The exact molecular function of Wdr13 is still largely unknown. In order to find out the role of Wdr13 in brain, we chose to characterize Wdr13 gene knockout mice and dissect the molecular mechanisms behind its action. We found that Wdr13 expresses majorly in hippocampal formation, cerebral cortex, cerebellum and striatum. Wdr13-/0 mice show mild anxiety but fared better than the wild type mice in memory tests. This phenotype was found to be correlated with increase in level of synaptic genes like Syn1, Camk2a, Sv2b and voltage gated ion channels. Interestingly, exposure to three/four weeks of social isolation caused symptoms of major depressive disorder in the Wdr13-/0 mice which was associated with loss in dendritic branches in hippocampal CA1 neurons and significant decrease in synaptic genes like Syn1, Rab3a, Nrxn2. We found GATA1, a common negative Transcription Factor for the above-mentioned and also a marker of MDD (Major Depressive Disorder) to be upregulated in the Wdr13-/0 mice after social isolation. WDR13 overexpression caused decrease in GATA1 expression in IMR32- human neuroblastoma cell line indicating a possible regulation of GATA1 by WDR13. Wdr13 thus becomes an important candidate gene to be involved in Major Depression.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:19:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiladitya Mitra
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-02 00:46:21	ID requested
1	2017-01-03 00:01:21	announced
⏵ 2	2017-10-24 04:19:42	announced	Updated project metadata.

Mitra S, Sameer Kumar GS, Tiwari V, Lakshmi BJ, Thakur SS, Kumar S, Implication of Genetic Deletion of Wdr13 in Mice: Mild Anxiety, Better Performance in Spatial Memory Task, with Upregulation of Multiple Synaptic Proteins. Front Mol Neurosci, 9():73(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Wdr13-/0, mice, hippocampus, pre-frontal cortex, iTRAQ

Suman Thakur
contact affiliation	Centre for Cellular and Molecular Biology
contact email	sst@ccmb.res.in
lab head
Shiladitya Mitra
contact affiliation	Centre for Cellular and Molecular Biology
contact email	shiladitya.mitra@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002466
     1. Label: PRIDE project
     2. Name: Wdr13-/0 compared to wild-type mice hippocampus and pre-frontal cortex iTRAQ proteomics