PXD002455

PXD002455 is an original dataset announced via ProteomeXchange.

Title	Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.
Description	All vertebrates have multiple genes encoding for different CASQ isoforms. Increasing interest has been focused on mammalian and human CASQ genes since mutations of both cardiac (CASQ2) and skeletal (CASQ1) isoforms cause different, and sometime severe, human pathologies Danio rerio (zebrafish) is a powerful model for studying function and mutations of human proteins. In this work expression, biochemical properties and cellular and sub-cellular localization of Danio rerio native CASQ isoforms are investigated. By quantitative PCR three mRNAs were detected in skeletal muscle and one mRNA in heart. Three zebrafish CASQs were identified by mass spectrometry and they share properties with mammalian skeletal and cardiac CASQs. Skeletal calsequestrins were found primarily, but not exclusively, at the sarcomere Z-line level where Terminal Cisternae of Sarcoplasmic reticulum are located.
HostingRepository	PRIDE
AnnounceDate	2015-11-10
AnnouncementXML	Submission_2015-11-23_06:02:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagarjuna Nagaraj
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-07-01 00:03:39	ID requested
1	2015-11-10 06:24:10	announced
⏵ 2	2015-11-23 06:02:20	announced	Updated publication reference for PubMed record(s): 26585961.

Furlan S, Mosole S, Murgia M, Nagaraj N, Argenton F, Volpe P, Nori A, Calsequestrins in skeletal and cardiac muscle from adult Danio rerio. J Muscle Res Cell Motil, 37(1-2):27-39(2016) [pubmed]

submitter keyword: Calsequestrins, skeletal, cardiac, muscle

Alessandra Nori
contact affiliation	Department of Biomedical Sciences, University of Padova, Istituto Interuniversitario di Miologia, viale G. Colombo 3, 35121 Padova (Italy)
contact email	alessandra.nori@unipd.it
lab head
Nagarjuna Nagaraj
contact affiliation	MaxPlanck Institute for Biochemistry, Core facility
contact email	nagaraj@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/11/PXD002455

PRIDE project URI

• PRIDE
  1. PXD002455
     1. Label: PRIDE project
     2. Name: Calsequestrins in skeletal and cardiac muscle from adult Danio rerio.