PXD002441

PXD002441 is an original dataset announced via ProteomeXchange.

Title	Daphnia pulex_Lysine acetylation
Description	Daphnia (Daphnia pulex) is a small planktonic crustacean and a key constituent of aquatic ecosystems. It is commonly used as a model organism for studying environmental toxic challenges. In the past decade, a Daphnia genomic information and proteomic dataset has been developed. This dataset has expanded the opportunity to relate toxicological effects with “Daphnia proteomics” as it integrates proteomic knowledge in Daphnia, those approach will provide greater insights for toxicological research. In order to exploit Daphnia for ecotoxicological research, information on the post-translational modification (PTM) of proteins is necessary as this is a critical regulator of biological processes. Acetylation of lysine (Kac) is a reversible and highly regulated PTM that is associated with diverse biological functions. However, a comprehensive description of Kac in Daphnia is not yet available. Here, to understand the cellular distribution of lysine acetylation in Daphnia, we identified 98 acetylation sites in 65 proteins by immunoprecipitation using an anti-acetyllysine antibody and an liquid chromatography system supported by mass spectroscopy. We identified 28 acetylated sites connected with metabolic proteins and 6 acetylated enzymes associated with the TCA cycle in Daphnia. From GO and KEGG enrichment analyses, we showed that Kac in D. pulex is highly enriched in proteins associated with metabolic processes. Our data provide the first global analysis of lysine acetylation in D. pulex. The expanded proteomic dataset will be an important resource for the functional analysis of Kac in D. pulex and it will be nice to have a first step done using a promising future model organism.
HostingRepository	PRIDE
AnnounceDate	2016-01-05
AnnouncementXML	Submission_2016-01-05_14:31:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oh Kwang Kwon
SpeciesList	scientific name: Daphnia pulex (Water flea); NCBI TaxID: 6669;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-29 02:38:39	ID requested
⏵ 1	2016-01-05 14:31:52	announced

Kwon OK, Sim J, Kim SJ, Oh HR, Nam DH, Lee S, Global proteomic analysis of protein acetylation affecting metabolic regulation in Daphnia pulex. Biochimie, 121():219-27(2016) [pubmed]

curator keyword: Biological

submitter keyword: D. pulex

lysine acetylation

post-translational modification

proteomics

Sangkyu Lee
contact affiliation	College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University
contact email	sangkyu@knu.ac.kr
lab head
Oh Kwang Kwon
contact affiliation	Kyungpook National University
contact email	kok0100@nate.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002441
     1. Label: PRIDE project
     2. Name: Daphnia pulex_Lysine acetylation