PXD002426

PXD002426 is an original dataset announced via ProteomeXchange.

Title	Quantitative lipoproteomics in Clostridium difficile reveals a role for lipoproteins in sporulation
Description	Bacterial lipoproteins are surface exposed, anchored to the membrane by S diacylglyceryl modification of the N-terminal cysteine thiol. They play important roles in many essential cellular processes including, in bacterial pathogens, at the host-pathogen interface. Clostridium difficile is a Gram-positive anaerobe that causes severe gastrointestinal disease. Its lipoproteome remains poorly characterized, but is likely to be important in colonization of the host and in transmission of infection. This dataset utilised metabolic tagging with alkyne-tagged lipid analogues, followed by click ligation to biotin and affintiy enrichment, in combination with diemthyl albelling based quantitative proteomics to profile protein lipidation across diverse C. difficile strains. Additionally, the lipoproteomes of mutants inactivated in specific components of the lipoprotein biogenesis pathway and a mutant in the spo0A gene are included. For all strains a complementary global proteome is provided, split into soluble and insoluble fractions and quantifed by dimethyl labelling.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_03:00:49.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Charlton
SpeciesList	scientific name: Peptoclostridium difficile (strain R20291) (Clostridium difficile); NCBI TaxID: 645463; scientific name: Peptoclostridium difficile (strain 630) (Clostridium difficile); NCBI TaxID: 272563;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-25 07:47:05	ID requested
⏵ 1	2022-03-02 03:00:50	announced

Charlton TM, Kovacs-Simon A, Michell SL, Fairweather NF, Tate EW, Quantitative Lipoproteomics in Clostridium difficile Reveals a Role for Lipoproteins in Sporulation. Chem Biol, 22(11):1562-1573(2015) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Clostridium difficile, Peptoclostridium difficile, 630, R20291, YnMyr, lipoproteome

Edward W. Tate
contact affiliation	Imperial College London, United Kingdom
contact email	e.tate@imperial.ac.uk
lab head
Thomas Charlton
contact affiliation	Imperial College London
contact email	Thomas.Charlton10@imperial.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002426
     1. Label: PRIDE project
     2. Name: Quantitative lipoproteomics in Clostridium difficile reveals a role for lipoproteins in sporulation