PXD002408

PXD002408 is an original dataset announced via ProteomeXchange.

Title	Quantitative K-GG site analysis
Description	Post-translational modification of proteins by ubiquitin (UQ) and UQ-like modifiers is emerging as a central pathway in DNA replication. We previously showed that chromatin in the vicinity of replisomes is rich in SUMO and depleted in UQ, whereas an opposite pattern is observed in mature chromatin. How this SUMO-rich/UQ-low environment is maintained at replisomes is not known. Here we identify USP7 as a SUMO deubiquitinase that localizes to replication forks and is essential for DNA replication. By acting on SUMO and SUMOylated proteins, USP7 prevents their ubiquitination. Chemical inhibition or genetic deletion of USP7 leads to the accumulation of UQ on SUMOylated proteins, which are displaced from the replisomes. Our findings provide a model to explain the differential accumulation of SUMO and UQ in replication forks versus mature chromatin, and identify an essential role of USP7 that should be taken into account for the use of USP7 inhibitors as anticancer agents.
HostingRepository	PRIDE
AnnounceDate	2016-01-13
AnnouncementXML	Submission_2016-01-13_01:59:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Isabel Ruppen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-22 07:16:47	ID requested
⏵ 1	2016-01-13 01:59:34	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: USP7 inhibitor, ubiquitylation, LC-MS/MS

Oscar Fernández Capetillo
contact affiliation	Genomic Instability Group, Spanish National Cancer Research Center (CNIO)
contact email	ofernandez@cnio.es
lab head
Isabel Ruppen
contact affiliation	CNIO
contact email	miruppen@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/01/PXD002408

PRIDE project URI

• PRIDE
  1. PXD002408
     1. Label: PRIDE project
     2. Name: Quantitative K-GG site analysis