PXD002404

PXD002404 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic analysis of laboratory-scale floccular and granular biofilms enriched with Accumulibacter phosphatis
Description	Biofilms are ubiquitous in nature, forming diverse adherent microbial communities that perform a plethora of functions. Here, we operated two laboratory-scale sequence batch reactors enriched with Candidatus Accumulibacter phosphatis (Accumulibacter) performing enhanced biological phosphorus removal (EBPR). Reactors formed two distinct biofilms, a floccular biofilm, consisting of small, loose, microbial aggregates, and a granular biofilm, forming larger, dense, spherical aggregates. Using metaproteomic methods we investigated the proteomic differences between these two biofilm communities, identifying a total of 2022 unique proteins. Both biofilms contained proteins that were indicative of core EBPR metabolisms and cellular function. To understand the proteomic differences between floccular and granular biofilm communities, we compared protein abundances that were statistically enriched in both biofilm states (alpha level = 0.05). Floccular biofilms were enriched with pathogenic secretion systems suggesting a previously unrecognized, highly competitive, mixed microbial community. Comparatively, granular biofilms revealed a high stress environment with evidence of nutrient starvation, phage predation pressure, extracellular polymeric substance (EPS) synthesis, and increased cell lysis. Granular biofilms enriched outermembrane transport proteins to scavenge the extracellular milieu for amino acids and other metabolites, likely released through cell lysis, to supplement core EBPR metabolic pathways. This study provides the first detailed proteomic comparison between Accumulibacter–enriched floccular and granular biofilm communities, proposes a conceptual model for the granule biofilm, and offers novel insights into granule biofilm formation and stability.
HostingRepository	PRIDE
AnnounceDate	2015-08-24
AnnouncementXML	Submission_2015-08-24_06:07:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bas E. Dutilh
SpeciesList	scientific name: Candidatus Accumulibacter phosphatis; NCBI TaxID: 327160;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-22 02:04:10	ID requested
⏵ 1	2015-08-24 06:07:59	announced

Barr JJ, Dutilh BE, Skennerton CT, Fukushima T, Hastie ML, Gorman JJ, Tyson GW, Bond PL, Metagenomic and metaproteomic analyses of Accumulibacter phosphatis-enriched floccular and granular biofilm. Environ Microbiol, 18(1):273-87(2016) [pubmed]

curator keyword: Metaproteomics, Biological

submitter keyword: Accumulibacter phosphatis, wastewater, flocs, granules, CapHPLC-MS/MS

Philip L. Bond
contact affiliation	The University of Queensland, Advanced Water Management Centre (AWMC), QLD 4072, Australia
contact email	p.bond1@uq.edu.au
lab head
Bas E. Dutilh
contact affiliation	Utrecht University
contact email	bedutilh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002404
     1. Label: PRIDE project
     2. Name: Metaproteomic analysis of laboratory-scale floccular and granular biofilms enriched with Accumulibacter phosphatis