PXD002398

PXD002398 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of host-parasite interactions in Giardia duodenalis
Description	Giardia duodenalis is the protozoan agent responsible for the majority of parasitic gastroenteritis in humans worldwide. Disease pathology includes malabsorption and maldigestion, cell apoptosis and small intestinal barrier dysfunction, which occurs in absence of known toxins, cell invasion and overt inflammation. To understand pathogenesis, host-parasite in vitro interaction models provide global insights into disease induction and virulence. Hence, we performed the first proteomic analysis of G. duodenalis trophozoites interacted with intestinal epithelial cells (IECs, HT-29) for 6 hours, and compared it to trophozoites exposed to cell-free fractions of host-soluble signals. This has allowed us to demonstrate that distinct and independent protein cascades are induced by host attachment compared to host soluble signals. We utilised a tandem mass tag (TMT) approach and evaluated it as quantitative proteomics for the first time in Giardia.
HostingRepository	PRIDE
AnnounceDate	2016-02-19
AnnouncementXML	Submission_2016-02-19_05:34:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Samantha Emery
SpeciesList	scientific name: Giardia intestinalis assemblage A; NCBI TaxID: 941442;
ModificationList	deamidated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-19 03:31:22	ID requested
⏵ 1	2016-02-19 05:34:07	announced

Emery SJ, Mirzaei M, Vuong D, Pascovici D, Chick JM, Lacey E, Haynes PA, Induction of virulence factors in Giardia duodenalis independent of host attachment. Sci Rep, 6():20765(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: TMT labelling, quantitative proteomics, host-parasite interactions, parasitology, Giardia, Giardia duodenalis

Professor Paul Haynes
contact affiliation	Macquarie University, North Ryde, Sydney Australia Faculty of Science and Engineering Deparment of Chemistry and Biomolecular Science
contact email	paul.haynes@mq.edu.au
lab head
Samantha Emery
contact affiliation	Chemistry and Biomolecular Sciences
contact email	samantha.emery@mq.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002398

PRIDE project URI

• PRIDE
  1. PXD002398
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of host-parasite interactions in Giardia duodenalis