PXD002397

PXD002397 is an original dataset announced via ProteomeXchange.

Title	Helianthus annuus (sunflower) allergen identification by mass spectrometry
Description	Background: Respiratory allergy triggered by pollen allergens is increasing at an alarming rate worldwide. Sunflower pollen is thought to be an important source of inhalant allergens. Present study aims to identify the prevalence of sunflower pollinosis among the Indian allergic population and characterizes the pollen allergens using immuno-proteomic tools. Methodology: Clinico-immunological tests were performed to understand the prevalence of sensitivity towards sunflower pollen among the atopic population. Sera from selected sunflower positive patients were used as probe to detect the IgE-reactive proteins from the one and two dimensionally separated proteome of sunflower pollen. The antigenic nature of the sugar moiety of the glycoprotein allergens was studied by meta-periodate modification of IgE-immunoblot. Finally, these allergens were identified by mass-spectrometry (MALDI TOF/TOF and LC ESI qTOF). MASCOT searching was performed against NCBInr database. However, Helianthus annuus genome is not fully sequenced and partially annotated. So in case of low confidence (p> 0.05) protein identification, searching was performed against EST library of Helianthus annuus. Results: Prevalence of sunflower pollen allergy was observed among 21% of the atopic population and associated with elevated level of specific IgE and histamine in the sera of these patients. Immunoscreening of sunflower pollen proteome with patient serum detected seven IgE-reactive proteins with varying molecular weight and pI. Hierarchical clustering of 2D-immunoblot data highlighted three allergens characterized by a more frequent immuno-reactivity and increased levels of IgE antibodies in the sera of susceptible patients. These allergens were considered as the major allergens of sunflower pollen and were found to have their glycan moiety critical for inducing IgE response. Homology driven search of MS/MS data of these IgE-reactive proteins identified seven previously unreported allergens from sunflower pollen. Three major allergenic proteins were identified as two non-isoformic pectate lyases and a cystein protease. Conclusion: Novelty of the present report is the identification of a panel of seven sunflower pollen allergens for the first time at immuno-biochemical and proteomic level, which substantiated the clinical evidence of sunflower allergy. Further purification and recombinant expression of these allergens will improve component-resolved diagnosis and therapy of pollen allergy.
HostingRepository	PRIDE
AnnounceDate	2015-10-05
AnnouncementXML	Submission_2015-10-05_06:19:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nandini Ghosh
SpeciesList	scientific name: Helianthus annuus (Common sunflower); NCBI TaxID: 4232;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex; maXis

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-19 03:06:21	ID requested
⏵ 1	2015-10-05 06:19:36	announced

Ghosh N, Sircar G, Saha B, Pandey N, Gupta Bhattacharya S, Search for Allergens from the Pollen Proteome of Sunflower (Helianthus annuus L.): A Major Sensitizer for Respiratory Allergy Patients. PLoS One, 10(9):e0138992(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: MALDI TOF/TOF, LC ESI qTOF, NCBInr, EST library of Helianthus annuus, allergen

Swati Gupta Bhattacharya
contact affiliation	Senior Professor and Professor-in-Charge, Division of Plant Biology Bose Institute, Kolkata, India
contact email	swati@jcbose.ac.in
lab head
Nandini Ghosh
contact affiliation	Bose Institute, Kolkata, India
contact email	nandini.ghosh14@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002397
     1. Label: PRIDE project
     2. Name: Helianthus annuus (sunflower) allergen identification by mass spectrometry