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PXD002390

PXD002390 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of microRNA394 target genes in A. thaliana
DescriptionExpression of the F-Box protein Leaf Curling Responsiveness (LCR) is regulated by microRNA, miR394, and alterations to this interplay in Arabidopsis thaliana produce defects in leaf polarity and shoot apical meristem (SAM) organisation. Although the miR394-LCR node has been documented in Arabidopsis, the identification of proteins targeted by LCR F-box itself has proven problematic. Here, a proteomic analysis of shoot apices from plants with altered LCR levels identified a member of the Major Latex Protein (MLP) family gene as a potential LCR F-box target. Bioinformatic and molecular analyses also suggested that other MLP family members are likely to be targets for this post-translational regulation. Direct interaction between LCR F-Box and MLP423 was validated. Additional MLP members had reduction in protein accumulation, in varying degrees, mediated by LCR F-Box. Transgenic Arabidopsis lines, in which MLP28 expression was reduced through an artificial miRNA technology, displayed severe developmental defects, including changes in leaf patterning and morphology, shoot apex defects, and eventual premature death. These phenotypic characteristics resemble those of Arabidopsis plants modified to over-express LCR. Taken together, the results demonstrate that MLPs are driven to degradation by LCR, and indicate that MLP gene family is target of miR394-LCR regulatory node, representing potential targets for directly post-translational regulation mediated by LCR F-Box. In addition, MLP28 family member is associated with the LCR regulation that is critical for normal Arabidopsis development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:11:36.036.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBenjamin Parker
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-06-18 01:30:50ID requested
12016-07-19 04:34:43announced
22024-10-22 04:11:39announced2024-10-22: Updated project metadata.
Publication List
Litholdo CG, Parker BL, Eamens AL, Larsen MR, Cordwell SJ, Waterhouse PM, Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis thaliana Identifies Major Latex Protein Family Members Critical for Normal Development. Mol Cell Proteomics, 15(6):2033-47(2016) [pubmed]
10.1074/mcp.M115.053124;
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis,LC-MS/MS, plant development, MLP proteins, microRNA394, LCR F-Box, gene regulation
Contact List
Benjamin Parker
contact affiliationThe university of sydney
contact emailbenjamin.parker@sydney.edu.au
lab head
Benjamin Parker
contact affiliationThe University of Melbourne
contact emailben.parker@unimelb.edu.au
dataset submitter
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Dataset FTP location
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PRIDE project URI
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