PXD002384

PXD002384 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of the interplay of Staphylococcus aureus and human alveolar epithelial cells during infection - S. aureus proteins
Description	Since infectious diseases caused by Staphylococcus aureus are still a major threat for human health we aimed to gain a detailed understanding of the molecular interplay of pathogen and host upon internalization by proteome analyses. In the present study we infected human alveolar epithelial A549 cells with S. aureus HG001 pMV158GFP and separated intact bacteria from host cell debris or infected from non-infected A549 cells prior to proteome analysis by cell sorting thus facilitating detailed analyses. During the first 6.5 h in the intracellular milieu S. aureus displayed reduced growth rate, induction of the stringent response, and adaptation to microaerobic conditions as well as cell wall stress. Interestingly, both truly infected host cells and those only potentially exposed to secreted S. aureus proteins but not infected displayed differences in the proteome pattern compared to A549 cells which had never been in contact with S. aureus. However, adaptation reactions were more pronounced in infected compared to non-infected A549 cells. Additional cytokine measurements revealed elaborated levels of pro-inflammatory cytokines in supernatants of the infection setting compared to pure host cell cultures which might mediate communication among the host cells and trigger adaptation even in cells not infected with S. aureus.
HostingRepository	PRIDE
AnnounceDate	2015-08-05
AnnouncementXML	Submission_2015-08-10_16:11:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Surmann
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-17 06:59:26	ID requested
1	2015-08-05 06:10:03	announced
⏵ 2	2015-08-10 16:11:20	announced	Updated publication reference for PubMed record(s): 26244908.

Surmann K, Simon M, Hildebrandt P, Pf, ö, rtner H, Michalik S, Stentzel S, Steil L, Dhople VM, Bernhardt J, Schl, ü, ter R, Depke M, Gierok P, Lalk M, Br, ö, ker BM, Schmidt F, V, ö, lker U, A proteomic perspective of the interplay of Staphylococcus aureus and human alveolar epithelial cells during infection. J Proteomics, 128():203-17(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Staphylococcus aureus, epithelial cells, internalization, host-pathogen interaction, proteomics

Uwe Völker
contact affiliation	Functional Genomics, University Medicine Greifswald, Germany
contact email	voelker@uni-greifswald.de
lab head
Kristin Surmann
contact affiliation	University Medicine Greifswald
contact email	surmannk@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002384

PRIDE project URI

• PRIDE
  1. PXD002384
     1. Label: PRIDE project
     2. Name: Proteomic characterization of the interplay of Staphylococcus aureus and human alveolar epithelial cells during infection - S. aureus proteins