PXD002378

PXD002378 is an original dataset announced via ProteomeXchange.

Title	Quantitative cross-linking analysis of chemoresistant HeLa cells
Description	Chemoresistance is a common mode of therapy failure for many cancers. Tumors develop resistance to chemotherapeutics through a variety of mechanisms, with proteins serving pivotal roles. Changes in protein conformations and interactions affect the cellular response to environmental conditions contributing to the development of new phenotypes. The ability to understand how protein interaction networks adapt to yield new function or alter phenotype is limited by the inability to determine structural and protein interaction changes on a proteomic scale. In this project we combine SILAC with PIR cross-linking technology to generate a quantitative protein interaction map of drug senstive and drug resistant HeLa cells.
HostingRepository	PRIDE
AnnounceDate	2015-09-15
AnnouncementXML	Submission_2015-09-15_04:28:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juan Chavez
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N6: N6; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; monomethylated residue; dimethylated residue; 6x(13)C labeled L-arginine; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-17 01:19:43	ID requested
⏵ 1	2015-09-15 04:28:55	announced

Chavez JD, Schweppe DK, Eng JK, Zheng C, Taipale A, Zhang Y, Takara K, Bruce JE, Quantitative interactome analysis reveals a chemoresistant edgotype. Nat Commun, 6():7928(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Protein interactions, multidrug resistant cancer, chemical cross-linking, mass spectrometry

James E. Bruce
contact affiliation	Department of Genome Sciences, University of Washington
contact email	jimbruce@uw.edu
lab head
Juan Chavez
contact affiliation	University of Washington
contact email	jdchavez@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD002378

PRIDE project URI

• PRIDE
  1. PXD002378
     1. Label: PRIDE project
     2. Name: Quantitative cross-linking analysis of chemoresistant HeLa cells