<<< Full experiment listing

PXD002373

PXD002373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome and carbon flux analysis of Pseudomonas aeruginosa clinical isolates from different infection sites
DescriptionPseudomonas aeruginosa is known as opportunistic pathogen frequently isolated from different infection sites such as burned wounds, lung and urinary tract. To shed light on the expression rates of cytoplasmic P. aeruginosa proteins, commonly expressed by eleven different clinical isolates, absolute protein quantities were determined using P. aeruginosa PAO1 as a reference strain and employing a highly precise gel-free and data-independent LC-IMSE approach. Moreover, the metabolic diversity of these isolates has been investigated by 13C-metabolic flux analyses. 903 proteins were reproducibly identified and absolutely quantified for P. aeruginosa PAO1, 363 of which were also identified and relatively quantified in all of the tested clinical isolates. The vast majority of these proteins is expressed in constant amounts in all strains and exhibits a relatively low relative standard deviation. In contrast, the expression rates of 42 proteins were highly variable between the isolates. Notably, the outer membrane protein OprH and the response regulator PhoP were strongly expressed in isolates from burned wounds when compared to isolates from lung or urinary tract. Moreover, proteins involved in the uptake of iron and amino acids (i.e. HitA, BfrB, PA5217, BraC, PA5153) were found to be more abundant in urinary tract isolates compared to lung isolates. The fluxome data revealed a conserved glycolysis, and a niche-specific divergence in fluxes through the glyoxylate shunt and the TCA cycle among the isolates. The integrated analysis of proteome and fluxome did not indicate straightforward correlation between the amount of proteins and their flux, but rather points to additional layers of regulation that mediate metabolic adaption of P. aeruginosa to different host environments.
HostingRepositoryPRIDE
AnnounceDate2016-03-31
AnnouncementXMLSubmission_2016-03-31_04:52:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Lassek
SpeciesList scientific name: Pseudomonas aeruginosa; NCBI TaxID: 287;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-06-15 06:25:14ID requested
12016-03-31 04:52:36announced
Publication List
Lassek C, Berger A, Z, ΓΌ, hlke D, Wittmann C, Riedel K, aeruginosa clinical isolates from different infection sites. Proteomics, 16(9):1381-5(2016) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Clinical isolates, gel-free and label-free proteomics
Contact List
Katharina Riedel
contact affiliationUniversity of Greifswald, Institute of Microbiology, Greifswald, Germany
contact emailriedela@uni-greifswald.de
lab head
Christian Lassek
contact affiliationUniversity of Greifswald
contact emaillassekc@uni-greifswald.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/03/PXD002373
PRIDE project URI
Repository Record List
[ + ]