PXD002369

PXD002369 is an original dataset announced via ProteomeXchange.

Title	Selective labeling and eradication of antibiotic tolerant bacterial populations in pathogenic biofilms
Description	Drug resistance and tolerance eliminate the therapeutic potential of antibiotics against pathogens. Antibiotic tolerance by bacterial biofilms often leads to persistent infections, but its mechanisms are unclear. To uncover antibiotic tolerance mechanisms in biofilms, we applied stable isotope labeling with amino acids (SILAC) proteomics to selectively label and compare proteomes of sensitive and tolerant subpopulations of biofilms formed by Pseudomonas aeruginosa towards colistin, a 'last-resort' antibiotic against multidrug-resistant Gram-negative pathogens. Migration was essential in forming colistin-tolerant biofilm subpopulations, as colistin-tolerant cell-aggregates migrated with type IV pili, onto the top of killed biofilm. The colistin-tolerant cell-aggregates employed quorum sensing (QS) to initiate the formation of fresh colistin-tolerant subpopulations, highlighting multicellular behavior in antibiotic tolerance development. Erythromycin treatment which inhibits motility and QS, boosted biofilm eradication by colistin. This novel ‘-omics’ strategy to study antibiotic tolerant cells provides key insights for designing novel treatments against infections unsuppressed by conventional antimicrobials.
HostingRepository	PRIDE
AnnounceDate	2016-02-16
AnnouncementXML	Submission_2016-03-16_06:16:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sunil Adav
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1H2O; NCBI TaxID: 1147787;
ModificationList	deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-15 01:25:47	ID requested
1	2016-02-16 01:04:26	announced
⏵ 2	2016-03-16 06:16:13	announced	Updated publication reference for PubMed record(s): 26892159.

Chua SL, Yam JK, Hao P, Adav SS, Salido MM, Liu Y, Givskov M, Sze SK, Tolker-Nielsen T, Yang L, Selective labelling and eradication of antibiotic-tolerant bacterial populations in Pseudomonas aeruginosa biofilms. Nat Commun, 7():10750(2016) [pubmed]

submitter keyword: Biofilm

Siu Kwan Sze
contact affiliation	School of Biological Sciences, Nanyang Technological University, Singapore
contact email	sksze@ntu.edu.sg
lab head
Sunil Adav
contact affiliation	Nanyang Technological University
contact email	ssadav@ntu.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002369

PRIDE project URI

• PRIDE
  1. PXD002369
     1. Label: PRIDE project
     2. Name: Selective labeling and eradication of antibiotic tolerant bacterial populations in pathogenic biofilms