PXD002365

PXD002365 is an original dataset announced via ProteomeXchange.

Title	The Lipid Raft Proteome of Borrelia burgdorferi.
Description	Eukaryotic lipid rafts are membrane microdomains rich in cholesterol that contain a selective set of proteins, and have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol onto its membrane, and form cholesterol glycolipid domains that possess all the hallmarks of eukaryotic lipid rafts. In this study, we isolated lipid rafts from cultured B. burgdorferi as a detergent resistant membrane (DRM) fraction on density gradients, and characterized those molecules that partitioned exclusively or are highly enriched in these domains. Cholesterol glycolipids, the previously known raft-associated lipoproteins OspA and OpsB, and cholera toxin partitioned into the lipid rafts fraction indicating compatibility with components of the DRM. The proteome of lipid rafts was analyzed by a combination of LC-MS/MS or MudPIT. Identified proteins were analyzed in silico for parameters that included localization, isoelectric point, molecular mass and biological function. The proteome provided a consistent pattern of lipoproteins, proteases and their substrates, sensing molecules, and prokaryotic homologs of eukaryotic lipid rafts. This study provides the first analysis of a prokaryotic lipid raft and has relevance for the biology of Borrelia, other pathogenic bacteria, as well as for the evolution of these structures.
HostingRepository	PRIDE
AnnounceDate	2015-09-21
AnnouncementXML	Submission_2015-09-21_04:34:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002365
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alvaro Toledo
SpeciesList	scientific name: Borrelia burgdorferi B31; NCBI TaxID: 224326;
ModificationList	Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-12 01:59:03	ID requested
⏵ 1	2015-09-21 04:34:44	announced

Toledo A, P, é, rez A, Coleman JL, Benach JL, The lipid raft proteome of Borrelia burgdorferi. Proteomics, 15(21):3662-75(2015) [pubmed]

curator keyword: Biological

submitter keyword: bacteria, lipid raft, borrelia, prokaryotes, lyme disease

Jorge Benach
contact affiliation	Stony Brook University
contact email	jorge.benach@stonybrook.edu
lab head
Alvaro Toledo
contact affiliation	Stony Brook University
contact email	alvaro.m.toledo@stonybrook.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002365
     1. Label: PRIDE project
     2. Name: The Lipid Raft Proteome of Borrelia burgdorferi.