PXD002365 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Lipid Raft Proteome of Borrelia burgdorferi. |
Description | Eukaryotic lipid rafts are membrane microdomains rich in cholesterol that contain a selective set of proteins, and have been associated with multiple biological functions. The Lyme disease agent, Borrelia burgdorferi, is one of an increasing number of bacterial pathogens that incorporates cholesterol onto its membrane, and form cholesterol glycolipid domains that possess all the hallmarks of eukaryotic lipid rafts. In this study, we isolated lipid rafts from cultured B. burgdorferi as a detergent resistant membrane (DRM) fraction on density gradients, and characterized those molecules that partitioned exclusively or are highly enriched in these domains. Cholesterol glycolipids, the previously known raft-associated lipoproteins OspA and OpsB, and cholera toxin partitioned into the lipid rafts fraction indicating compatibility with components of the DRM. The proteome of lipid rafts was analyzed by a combination of LC-MS/MS or MudPIT. Identified proteins were analyzed in silico for parameters that included localization, isoelectric point, molecular mass and biological function. The proteome provided a consistent pattern of lipoproteins, proteases and their substrates, sensing molecules, and prokaryotic homologs of eukaryotic lipid rafts. This study provides the first analysis of a prokaryotic lipid raft and has relevance for the biology of Borrelia, other pathogenic bacteria, as well as for the evolution of these structures. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-21 |
AnnouncementXML | Submission_2015-09-21_04:34:43.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002365 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alvaro Toledo |
SpeciesList | scientific name: Borrelia burgdorferi B31; NCBI TaxID: 224326; |
ModificationList | Carbamidomethyl |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-06-12 01:59:03 | ID requested | |
⏵ 1 | 2015-09-21 04:34:44 | announced | |
Publication List
Toledo A, P, é, rez A, Coleman JL, Benach JL, The lipid raft proteome of Borrelia burgdorferi. Proteomics, 15(21):3662-75(2015) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: bacteria, lipid raft, borrelia, prokaryotes, lyme disease |
Contact List
Jorge Benach |
contact affiliation | Stony Brook University |
contact email | jorge.benach@stonybrook.edu |
lab head | |
Alvaro Toledo |
contact affiliation | Stony Brook University |
contact email | alvaro.m.toledo@stonybrook.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002365
- Label: PRIDE project
- Name: The Lipid Raft Proteome of Borrelia burgdorferi.