PXD002364

PXD002364 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteome of Magnaporthe oryzae
Description	Rice blast caused by Magnaporthe oryzae is the most devastating disease of cultivated rice. Several protein kinase cascades have been known to be essential to pathogenesis or important for response to stress, mycelial growth and conidiation in M. oryzae. However, phosphoproteins and their phosphorylation sites (p-sites) in this important fungal pathogen remain largely to be identified. In this study, 8087 phosphopeptides corresponding to 9825 p-sites from 1147 phosphoproteins were identified in mycelia of M. oryzae under a false discovery rate of < 0.55% at the peptide level. Notably, 33 previously reported pathogenesis-related proteins were included in the phosphoproteins at the mascot delta score  10. Further analyses of 581 motif-containing phosphoproteins that met more stringent criteria revealed that the phosphoproteins shared 19 distinct phosphorylation motifs, including the motif RxxpSP that was newly identified in this study but is widely distributed in diverse organisms. These phosphoproteins were mapped into 81 biological pathways. A total of 82 acidic motif-containing phosphoproteins were identified. Surprisingly, none of them except one were mapped to any of the metabolic pathways. Furthermore, a prediction disclosed a total of 174 kinase-substrate specific interactions in mycelia of M. oryzae. This study also detected phosphorylation of the tyrosine phosphatase Pmp1 and 7 other proteins upstream of Pmk1, but not Pmk1 and its downstream transcription factors. These results prompted a necessary revision of Pmk1 MAPK cascade, in which dephosphorylation of Pmk1 by Pmp1 in mycelia may block the activation of downstream targets.
HostingRepository	PRIDE
AnnounceDate	2017-07-17
AnnouncementXML	Submission_2017-07-17_06:11:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002364
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ruijin Wang
SpeciesList	scientific name: Magnaporthe oryzae (strain P131) (Rice blast fungus) (Pyricularia oryzae); NCBI TaxID: 1143193;
ModificationList	monohydroxylated residue: 15.994915; phosphorylated residue: 79.966331; iodoacetamide derivatized residue: 57.021464
Instrument	amaZon Speed ETD

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-12 01:12:21	ID requested
⏵ 1	2017-07-17 06:11:07	announced

Wang RJ, Peng J, Li QX, Peng YL, Revealed by Phosphoproteomic Analyses. Mol Cell Proteomics, 16(9):1669-1682(2017) [pubmed]

curator keyword: Biological

submitter keyword: Rice blast fungus, phosphorylation, mycelium, pathogensis

Qing X. Li
contact affiliation	Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, Hawaii 96822, USA
contact email	qingl@hawaii.edu
lab head
Ruijin Wang
contact affiliation	University of Hawaii/ China Agricultural University
contact email	wrjtjbd@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002364
     1. Label: PRIDE project
     2. Name: Phosphoproteome of Magnaporthe oryzae