PXD002353 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative proteomics Reveals Targets of Protein Kinase SnRK1 in Arabidopsis under Submergence |
Description | SNF1 RELATED PROTEIN KINASE 1 (SnRK1) is proposed as a central integrator of regulatory pathways in plant stress and energy starvation signaling. We observed in this study that the Arabidopsis SnRK1.1 dominant negative mutant (SnRK1.1K48M) had lower tolerance to submergence than the wild-type, suggesting that SnRK1.1-dependent phosphorylation of target proteins is important in energy starvation signaling triggered by submergence. To gain further insight into submergence signaling mechanisms, we determined the temporal response to energy starvation through AMP/ATP quantification and used quantitative phosphoproteomics to compare the global changes in phosphopeptides in Col-0 and SnRK1.1K48M. We found that the phosphorylation levels of 59 peptides increased and the levels of 96 peptides decreased in Col-0 within 0.5�� h of submergence. Among the 59 peptides with increased phosphorylation in Col-0, 49 did not show increased phosphorylation levels in SnRK1.1K48M under submergence. These proteins are involved in sugar synthesis, glycolysis, osmotic regulation, ABA signaling, protein synthesis and ROS signaling. In particular, the phosphorylation of MAPK6, which is involved in regulating ROS responses under different abiotic stresses, was disrupted in the SnRK1.1K48M mutant. In addition, PTP1, a negative regulator of MAPK6 activity that directly dephosphorylates MAPK6, was also regulated by SnRK1.1. These results reveal insights into the function of SnRK1 and the downstream signaling factors of submergence. |
HostingRepository | PRIDE |
AnnounceDate | 2016-04-07 |
AnnouncementXML | Submission_2016-04-07_03:49:36.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hsing Yi Cho |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | phosphorylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-06-11 06:58:55 | ID requested | |
⏵ 1 | 2016-04-07 03:49:37 | announced | |
Publication List
Cho HY, Wen TN, Wang YT, Shih MC, Quantitative phosphoproteomics of protein kinase SnRK1 regulated protein phosphorylation in Arabidopsis under submergence. J Exp Bot, 67(9):2745-60(2016) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: SnRK1.1, submergence, proteomics |
Contact List
Ming-Che Shih |
contact affiliation | Agricultural Biotechnology Research Center, Academia Sinica, Taiwan |
contact email | mcshih@gate.sinica.edu.tw |
lab head | |
Hsing Yi Cho |
contact affiliation | Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, National Chung-Hsing University, Academia Sinica, Taiwan |
contact email | hycho@gate.sinica.edu.tw |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002353
- Label: PRIDE project
- Name: Quantitative proteomics Reveals Targets of Protein Kinase SnRK1 in Arabidopsis under Submergence