PXD002347

PXD002347 is an original dataset announced via ProteomeXchange.

Title	Multiple sclerosis cuprizone mouse model and vitamin D supplementation
Description	Dietary supplementation of vitamin D is commonly recommended to patients with multiple sclerosis (MS). We investigated the effect of 1,25-dihydroxyvitamin-D3 (1,25-(OH)2D3) on the brain proteome in the cuprizone model during remyelination. Mice were demyelinated with dietary cuprizone for 7 weeks. The mice received intra-peritoneal injections of 1,25-(OH)2D3 or placebo twice a week, from week 6 and throughout week 10. Brain samples were taken after 7 weeks (demyelinated), after 8 weeks (1 week remyelination) and after 10 weeks (3 weeks of remyelination). The six experimental groups were labeled with TMT, mixed mode HPLC fractionation, and applied to LC-MS which enabled quantification of 5062 proteins with high confidence.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:09:38.063.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eystein Oveland
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-10 06:11:53	ID requested
1	2017-08-16 06:32:29	announced
2	2017-08-22 03:25:43	announced	Updated publication reference for PubMed record(s): 28818673.
⏵ 3	2024-10-22 04:09:38	announced	2024-10-22: Updated project metadata.

Oveland E, Nystad A, Berven F, Myhr KM, Torkildsen Ø, Wergeland S, 1,25-Dihydroxyvitamin-D3 induces brain proteomic changes in cuprizone mice during remyelination involving calcium proteins. Neurochem Int, 112():267-277(2018) [pubmed]

10.1016/j.neuint.2017.08.008;

curator keyword: Biomedical

submitter keyword: Mouse, multiple sclerosis, vitamin D, cuprizone TMT

Frode Berven
contact affiliation	Proteomics Unit at University of Bergen
contact email	frode.berven@uib.no
lab head
Eystein Oveland
contact affiliation	Institute of Marine Research
contact email	eysteino@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD002347

PRIDE project URI

• PRIDE
  1. PXD002347
     1. Label: PRIDE project
     2. Name: Multiple sclerosis cuprizone mouse model and vitamin D supplementation