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PXD002345

PXD002345 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChanges in the ascending aorta proteome of cholesterol-fed rabbits
DescriptionHere we undertook a proteomic investigation of ascending aorta from New Zealand White rabbits after 10 weeks on a high (2% w/w) cholesterol diet (HCD, n=5) or control diet (n=5) in order to profile the proteomic changes in response to the HCD. Histology confirmed intimal thickening in the HCD group and LC-MS/MS analysis of individually obtained ascending aorta extracts labelled with isobaric (iTRAQ) tags led to identification and quantitation of 453 unique proteins above the 1% false discovery rate threshold. Of 67 proteins showing significant differences in relative abundance (p<0.05), 62 were elevated and five decreased in ascending aorta from HCD-fed rabbits compared to controls. Six proteins were selected for validation using Multiple Reaction Monitoring which confirmed the iTRAQ results.
HostingRepositoryPRIDE
AnnounceDate2018-06-21
AnnouncementXMLSubmission_2018-06-21_04:09:34.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartin Middleditch
SpeciesList scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986;
ModificationListiTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQSTAR
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-06-10 03:13:47ID requested
12018-06-21 03:15:58announced
22018-06-21 04:09:35announcedUpdated publication reference for PubMed record(s): 26232167.
Publication List
Xu J, J, ΓΌ, llig M, Middleditch MJ, Cooper GJ, Modelling atherosclerosis by proteomics: Molecular changes in the ascending aortas of cholesterol-fed rabbits. Atherosclerosis, 242(1):268-76(2015) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: ascending aorta rabbit cholesterol itraq atherosclerosis
Contact List
Garth J.S. Cooper
contact affiliationUniversity of Auckland
contact emailg.cooper@auckland.ac.nz
lab head
Martin Middleditch
contact affiliationUniversity of Auckland
contact emailm.middleditch@auckland.ac.nz
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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