PXD002345 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Changes in the ascending aorta proteome of cholesterol-fed rabbits |
Description | Here we undertook a proteomic investigation of ascending aorta from New Zealand White rabbits after 10 weeks on a high (2% w/w) cholesterol diet (HCD, n=5) or control diet (n=5) in order to profile the proteomic changes in response to the HCD. Histology confirmed intimal thickening in the HCD group and LC-MS/MS analysis of individually obtained ascending aorta extracts labelled with isobaric (iTRAQ) tags led to identification and quantitation of 453 unique proteins above the 1% false discovery rate threshold. Of 67 proteins showing significant differences in relative abundance (p<0.05), 62 were elevated and five decreased in ascending aorta from HCD-fed rabbits compared to controls. Six proteins were selected for validation using Multiple Reaction Monitoring which confirmed the iTRAQ results. |
HostingRepository | PRIDE |
AnnounceDate | 2018-06-21 |
AnnouncementXML | Submission_2018-06-21_04:09:34.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Martin Middleditch |
SpeciesList | scientific name: Oryctolagus cuniculus (Rabbit); NCBI TaxID: 9986; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | QSTAR |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-06-10 03:13:47 | ID requested | |
1 | 2018-06-21 03:15:58 | announced | |
⏵ 2 | 2018-06-21 04:09:35 | announced | Updated publication reference for PubMed record(s): 26232167. |
Publication List
Xu J, J, ΓΌ, llig M, Middleditch MJ, Cooper GJ, Modelling atherosclerosis by proteomics: Molecular changes in the ascending aortas of cholesterol-fed rabbits. Atherosclerosis, 242(1):268-76(2015) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: ascending aorta rabbit cholesterol itraq atherosclerosis |
Contact List
Garth J.S. Cooper |
contact affiliation | University of Auckland |
contact email | g.cooper@auckland.ac.nz |
lab head | |
Martin Middleditch |
contact affiliation | University of Auckland |
contact email | m.middleditch@auckland.ac.nz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD002345 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002345
- Label: PRIDE project
- Name: Changes in the ascending aorta proteome of cholesterol-fed rabbits