PXD002317

PXD002317 is an original dataset announced via ProteomeXchange.

Title	wt vs daf-2 C. elegans protein turnover
Description	Most aging hypotheses revolve around the accumulation of some sort of damage resulting in gradual physiological decline and ultimately death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend lifespan. However, lowering translational efficiency extends rather than shortens lifespan in C. elegans. We studied turnover of individual proteins in the conserved Insulin/Insulin-like Growth Factor (IGF-1) receptor mutant daf-2 by combining Stable Isotope Labeling by Nitrogen-15 in Caenorhabditis elegans and LC-MS/MS. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, while others remained unchanged, signifying that longevity is not supported by high protein turnover. This slow-down of protein turnover was most prominent for components of the translation machinery and mitochondria. In contrast, the high turnover of lysosomal hydrolases and very low turnover of cytoskeletal proteins remained largely unchanged in daf-2. The slow-down of protein dynamics and decreased abundance of the translational machinery may point at the importance of anabolic attenuation in lifespan extension as suggested by the hyperfunction theory.
HostingRepository	PRIDE
AnnounceDate	2016-09-27
AnnouncementXML	Submission_2016-09-27_00:45:58.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002317
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vlad Petyuk
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-08 01:28:35	ID requested
⏵ 1	2016-09-27 00:45:59	announced

Dhondt I, Petyuk VA, Cai H, Vandemeulebroucke L, Vierstraete A, Smith RD, Depuydt G, Braeckman BP, elegans. Cell Rep, 16(11):3028-3040(2016) [pubmed]

curator keyword: Biological

submitter keyword: C. elegans, turnover, N15

Vlad Petyuk
contact affiliation	Pacific Northwest National Laboratory
contact email	vladislav.petyuk@pnnl.gov
lab head
Vlad Petyuk
contact affiliation	Pacific Northwest National Lab
contact email	vladislav.petyuk@pnnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002317
     1. Label: PRIDE project
     2. Name: wt vs daf-2 C. elegans protein turnover