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PXD002296

PXD002296 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBinding partners to Testis Specific Antigen, TSA70
DescriptionA testis specific antigen, TSA70 was identified in the lab which was homolgous to the sperm tail outer dense fiber protein (ODF2). ODFs are disulphide stabilised during epididymal maturation and are required for sperm motility. ODF2 is reported to bind macrophage migration inhibitory factor (MIF), a moonlighting oxidoreductase. It is not known whether such proteins exist in the female reproductive tract that ensure the disulphide stability. The aim was to identify full quorum of such redundant proteins in both male and female reproductive tract fluids. Using recombinant testis specific antigen, TSA70/ODF2, as bait we report the identification of binding partners from the epididymal fluid. Since MIF is a 12.5kDa protein, we focussed only on the low molecular weight proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:24:27.322.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKaushiki Kadam
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-06-03 01:22:51ID requested
12020-01-22 23:48:57announced
22024-10-22 04:24:29announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.freeradbiomed.2019.12.031;
Kadam K, Khole VV, Ghosalkar K, Jagtap D, Yarramala DS, Ramachandran B, Thiol based mechanism internalises interacting partners to outer dense fibers in sperm. Free Radic Biol Med, 148():170-181(2020) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: rat, MIF, ODF2
Contact List
Kaushiki Kadam
contact affiliationNational Institute for Research in Reproductive Health (ICMR), J.M. Street, Parel, Mumbai-400012
contact emailkadamk@nirrh.res.in
lab head
Kaushiki Kadam
contact affiliationNational Institute for Research in Reproductive Health
contact emailkadamk@nirrh.res.in
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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