PXD002296

PXD002296 is an original dataset announced via ProteomeXchange.

Title	Binding partners to Testis Specific Antigen, TSA70
Description	A testis specific antigen, TSA70 was identified in the lab which was homolgous to the sperm tail outer dense fiber protein (ODF2). ODFs are disulphide stabilised during epididymal maturation and are required for sperm motility. ODF2 is reported to bind macrophage migration inhibitory factor (MIF), a moonlighting oxidoreductase. It is not known whether such proteins exist in the female reproductive tract that ensure the disulphide stability. The aim was to identify full quorum of such redundant proteins in both male and female reproductive tract fluids. Using recombinant testis specific antigen, TSA70/ODF2, as bait we report the identification of binding partners from the epididymal fluid. Since MIF is a 12.5kDa protein, we focussed only on the low molecular weight proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:24:27.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kaushiki Kadam
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-03 01:22:51	ID requested
1	2020-01-22 23:48:57	announced
⏵ 2	2024-10-22 04:24:29	announced	2024-10-22: Updated project metadata.

10.1016/j.freeradbiomed.2019.12.031;

Kadam K, Khole VV, Ghosalkar K, Jagtap D, Yarramala DS, Ramachandran B, Thiol based mechanism internalises interacting partners to outer dense fibers in sperm. Free Radic Biol Med, 148():170-181(2020) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: rat, MIF, ODF2

Kaushiki Kadam
contact affiliation	National Institute for Research in Reproductive Health (ICMR), J.M. Street, Parel, Mumbai-400012
contact email	kadamk@nirrh.res.in
lab head
Kaushiki Kadam
contact affiliation	National Institute for Research in Reproductive Health
contact email	kadamk@nirrh.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD002296

PRIDE project URI

• PRIDE
  1. PXD002296
     1. Label: PRIDE project
     2. Name: Binding partners to Testis Specific Antigen, TSA70