PXD002296 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Binding partners to Testis Specific Antigen, TSA70 |
Description | A testis specific antigen, TSA70 was identified in the lab which was homolgous to the sperm tail outer dense fiber protein (ODF2). ODFs are disulphide stabilised during epididymal maturation and are required for sperm motility. ODF2 is reported to bind macrophage migration inhibitory factor (MIF), a moonlighting oxidoreductase. It is not known whether such proteins exist in the female reproductive tract that ensure the disulphide stability. The aim was to identify full quorum of such redundant proteins in both male and female reproductive tract fluids. Using recombinant testis specific antigen, TSA70/ODF2, as bait we report the identification of binding partners from the epididymal fluid. Since MIF is a 12.5kDa protein, we focussed only on the low molecular weight proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:24:27.322.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kaushiki Kadam |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-06-03 01:22:51 | ID requested | |
1 | 2020-01-22 23:48:57 | announced | |
⏵ 2 | 2024-10-22 04:24:29 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1016/j.freeradbiomed.2019.12.031; |
Kadam K, Khole VV, Ghosalkar K, Jagtap D, Yarramala DS, Ramachandran B, Thiol based mechanism internalises interacting partners to outer dense fibers in sperm. Free Radic Biol Med, 148():170-181(2020) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: rat, MIF, ODF2 |
Contact List
Kaushiki Kadam |
contact affiliation | National Institute for Research in Reproductive Health (ICMR), J.M. Street, Parel, Mumbai-400012 |
contact email | kadamk@nirrh.res.in |
lab head | |
Kaushiki Kadam |
contact affiliation | National Institute for Research in Reproductive Health |
contact email | kadamk@nirrh.res.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002296
- Label: PRIDE project
- Name: Binding partners to Testis Specific Antigen, TSA70