PXD002295

PXD002295 is an original dataset announced via ProteomeXchange.

Title	Poor preservation of antibodies in archaeological human bone and dentine
Description	The growth of proteomics-based methods in archaeology prompted an investigation of the survival of non-collagenous proteins, specifically immunoglobulin G (IgG), in archaeological human bone and dentine. Over a decade ago reports were published on extracted, immunoreactive archaeological IgG, and the variable yields of IgG molecules detected by Western blots of 1D and 2D SDS-PAGE gels. If IgG can indeed be recovered from archaeological skeletal material, it offers remarkable opportunities for exploring the history of disease - for example in applying functional anti-malarial IgGs to study past patterns of malaria. More recently, the field has seen a move away from immunological approaches and towards the use of shotgun proteomics via mass spectrometry. Using previously published techniques, this study attempted to extract and characterize archaeological IgG proteins. In only one extraction method were immunoglobulin derived peptides identified, and these displayed extensive evidence of degradation. The failure to extract immunoglobulins by all but one method, along with observed patterns of protein degradation, suggests that IgG may be an unsuitable target for detecting disease-associated antigens. This research highlights the importance of revisiting previously ‘successful’ biomolecular methodologies using emerging technologies.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:39:26.316.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Hendy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	hydroxylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	QSTAR; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-03 01:19:49	ID requested
1	2016-02-08 09:14:47	announced
2	2017-07-20 03:57:31	announced	Updated publication reference for DOI(s): 10.1080/20548923.2015.1133117.
⏵ 3	2023-11-14 06:39:26	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Technical

submitter keyword: Human, Dentine, Antibodies, Bone, Immunoglobulin, LC-MS/MS

Matthew Collins
contact affiliation	BioArCh, University of York, UK
contact email	matthew.collins@york.ac.uk
lab head
Jessica Hendy
contact affiliation	University of York
contact email	jessica.hendy@york.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/02/PXD002295

PRIDE project URI

• PRIDE
  1. PXD002295
     1. Label: PRIDE project
     2. Name: Poor preservation of antibodies in archaeological human bone and dentine