PXD002294

PXD002294 is an original dataset announced via ProteomeXchange.

Title	Updating the Proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs
Description	Mycoplasma suis belongs to the hemotrophic mycoplasma (HM) that are associated with acute and chronic anemia in a wide range of livestock and wild animals. The inability to culture HMs in vitro has hindered their characterization at the molecular level. Although genome sequences of eight HMs are available, there is only one study based on genome sequenced data on the proteomic level for HMs, namely M. suis. In the present work, the proteome of M. suis strain KI_3806 during acute infection was extended significantly by applying three different protein extraction methods, 1D SDS-PAGE and LC-MS/MS. A total of 404 of the 795 M. suis KI_3806 proteins (50.8%) of all encoded proteins were identified. Data analysis revealed the expression of 83.7% of the predicted ORFs with assigned functions but also highlights the expression of 179 of 523 (34.2%) hypothetical proteins with unknown functions. Computational analyses identified expressed membrane-associated hypothetical proteins that might be involved in adhesion or host-pathogen interaction. Furthermore, analyses of the expressed transporters indicated the existence of a hexose-6-phosphate-transporter and an ECF transporter. In conclusion, our proteome study provides a further step toward the elucidation of the unique life cycle of M. suis and the establishment of an in vitro culture.
HostingRepository	PRIDE
AnnounceDate	2015-12-17
AnnouncementXML	Submission_2016-01-04_11:32:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Lassek
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Mycoplasma suis (strain KI_3806); NCBI TaxID: 708248;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-02 06:39:16	ID requested
1	2015-12-17 07:56:39	announced
⏵ 2	2016-01-04 11:32:46	announced	Updated publication reference for PubMed record(s): 26678042.

Dietz S, Lassek C, Mack SL, Ritzmann M, Stadler J, Becher D, Hoelzle K, Riedel K, Hoelzle LE, Updating the proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs. Proteomics, 16(4):609-13(2016) [pubmed]

curator keyword: Biological

submitter keyword: Mycoplasma suis

Ludwig E. Hölzle
contact affiliation	Environmental and Animal Health, Universtity of Hohenheim, Germany
contact email	ludwig.hoelzle@uni-hohenheim.de
lab head
Christian Lassek
contact affiliation	University of Greifswald
contact email	lassekc@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002294
     1. Label: PRIDE project
     2. Name: Updating the Proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs