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PXD002292

PXD002292 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of secretomes from brain microvascular endothelial cells after ischemia
DescriptionThe human immortalized brain endothelial cell line hCMEC/D3 is considered an in vitro model of the blood-brain-barrier. We aimed to characterize changes in the secretome of hCMEC/D3 subjected to oxygen and glucose deprivation (OGD) by SILAC, in order to identify new proteins involved in ischemia-triggered blood-brain-barrier disruption and test their potential as blood biomarkers for ischemic stroke diagnosis. After SILAC analysis, 19 proteins were found differentially secreted between OGD and normoxia/normoglycemia conditions (Fold Change>|1.4| and peptide count≥2). Protein folding and nucleic acid binding were the main molecular functions and epithelial adherens junctions and aldosterone signaling appeared as the main canonical pathways represented by OGD-secreted proteins. ANXA1, CLUS, IGFBP2, PRDX3, TIMP2 and COL1A2 were replicated by western blotting in 9 independent cell cultures. Five replicated proteins were analyzed in human serum samples of 38 ischemic stroke patients compared to 18 stroke-mimicking conditions and 18 healthy controls by ELISA. IGFBP2 showed increased blood levels when strokes were compared with stroke-mimicking patients (p<0.1). In conclusion, we characterized changes in the secretome of hCMEC/D3 after an ischemic insult and highlighted some candidates to become biomarkers for ischemic stroke diagnosis related to blood-brain-barrier disruption.
HostingRepositoryPRIDE
AnnounceDate2016-01-21
AnnouncementXMLSubmission_2016-01-21_03:20:57.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002292
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVíctor Llombart
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList6x(13)C: 4x(15)N labeled L-arginine; monohydroxylated residue; iodoacetamide derivatized residue; 6x(13)C labeled residue
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-06-02 05:50:46ID requested
12016-01-21 03:20:58announced
Publication List
Llombart V, Garc, í, a-Berrocoso T, Bech-Serra JJ, Simats A, Bustamante A, Giralt D, Reverter-Branchat G, Canals F, Hern, á, ndez-Guillamon M, Montaner J, Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC). J Proteomics, 133():100-112(2016) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: SILAC, secretome, endothelial cells, oxygen and glucose deprivation, hCMEC/D3
Contact List
Joan Montaner
contact affiliationVall Hebron Institute of Research
contact emailjoan.montaner@vhir.org
lab head
Víctor Llombart
contact affiliationVall Hebrón Institute of Research
contact emailllombs@hotmail.com
dataset submitter
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Dataset FTP location
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