PXD002286

PXD002286 is an original dataset announced via ProteomeXchange.

Title	Phospo-proteomic profiling of Castration Resistant Prostate Cancer
Description	The integration of diverse ‘omic’ datasets will increase our understanding of the key signaling pathways that drive disease. Here, we used clinical tissue cohorts corresponding to lethal metastatic castration resistant prostate cancer (CRPC) obtained at rapid autopsy to integrate mutational, transcriptomic, and phosphoproteomic datasets for pathway analysis. Using Tied Diffusion through Interacting Events (TieDIE), we integrated differentially expressed transcriptional master regulators, functionally mutated genes, and differentially ‘activated’ kinases in CRPC tissues to synthesize a robust signaling network consisting of pathways with known and novel gene interactions. For 6 individual CRPC patients for which we had transcriptomic and phosphoproteomic data we observed distinct pathway activation states for each patient profile. In one patient, the activated pathways were strikingly similar to a prostate cancer cell line, 22Rv1, providing us with a good pre-clinical model to test targeted, combination therapies. In all, synthesis of multiple ‘omic’ datasets revealed a plethora of pathway information suitable for targeted therapies in lethal prostate cancer.
HostingRepository	PRIDE
AnnounceDate	2016-08-19
AnnouncementXML	Submission_2016-08-22_11:38:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nicholas Graham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2015-06-02 01:38:01	ID requested
1	2016-08-19 04:37:10	announced
⏵ 2	2016-08-22 11:38:20	announced	Updated publication reference for PubMed record(s): 27499020.

Drake JM, Paull EO, Graham NA, Lee JK, Smith BA, Titz B, Stoyanova T, Faltermeier CM, Uzunangelov V, Carlin DE, Fleming DT, Wong CK, Newton Y, Sudha S, Vashisht AA, Huang J, Wohlschlegel JA, Graeber TG, Witte ON, Stuart JM, Phosphoproteome Integration Reveals Patient-Specific Networks in Prostate Cancer. Cell, 166(4):1041-1054(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: phosphorylation, prostate cancer, human

Thomas Graeber
contact affiliation	David Geffen School of Medicine, University of California, Los Angeles, CA 90095 Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, Institute for Molecular Medicine, California NanoSystems Institute
contact email	tgraeber@mednet.ucla.edu
lab head
Nicholas Graham
contact affiliation	UCLA
contact email	ngraham379@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD002286

PRIDE project URI

• PRIDE
  1. PXD002286
     1. Label: PRIDE project
     2. Name: Phospo-proteomic profiling of Castration Resistant Prostate Cancer