PXD002265

PXD002265 is an original dataset announced via ProteomeXchange.

Title	Active Site Specificity Profiling of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by MMPs 1, 2, 3, 7, 8, 9, 12, 13, and 14.
Description	Background: The active site of matrix metalloproteinases (MMPs) is highly conserved, complicating the rational design of specific substrates and inhibitors for individual family members. Results: Active site specificity profiles of 9 MMPs were determined using high throughput Proteomic Identification of protease Cleavage Sites (PICS). Conclusion: Subtle specificity divergences distinguish individual MMP family members. Significance: Understanding individual MMP cleavage site specificities will bolster the design of MMP specific activity assays and targeted inhibitory drugs.
HostingRepository	PRIDE
AnnounceDate	2015-09-29
AnnouncementXML	Submission_2015-09-29_06:09:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich ECKHARD
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; N6: N6-dimethyl-L-lysine; (R)-5-oxo-1: 4-tetrahydrothiazine-3-carboxylic acid; thioacylation of primary amines - site N-term; alpha-amino dimethylated residue
Instrument	QSTAR Pulsar; QSTAR XL

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-29 09:22:25	ID requested
⏵ 1	2015-09-29 06:09:39	announced

Eckhard U, Huesgen PF, Schilling O, Bellac CL, Butler GS, Cox JH, Dufour A, Goebeler V, Kappelhoff R, Keller UAD, Klein T, Lange PF, Marino G, Morrison CJ, Prudova A, Rodriguez D, Starr AE, Wang Y, Overall CM, Active site specificity profiling of the matrix metalloproteinase family: Proteomic identification of 4300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses. Matrix Biol, 49():37-60(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: specificity profiling, MMPs, ECM, PICS, WebPICS, mass spectrometry

Prof. Christopher Mark Overall
contact affiliation	Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada
contact email	chris.overall@ubc.ca
lab head
Ulrich ECKHARD
contact affiliation	University of British Columbia
contact email	ulrich.eckhard@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/09/PXD002265

PRIDE project URI

• PRIDE
  1. PXD002265
     1. Label: PRIDE project
     2. Name: Active Site Specificity Profiling of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by MMPs 1, 2, 3, 7, 8, 9, 12, 13, and 14.