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PXD002264

PXD002264 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman Dental Pulp TAILS N-terminomics
DescriptionWe report the targeted analysis of the human dental pulp proteome and N-terminome using the positional proteomics technique TAILS, which allowed us to capture both naturally blocked and unblocked protein N-termini. Furthermore, by using a proteomically barely studied human tissue in combination with a non-shotgun proteomics approach, we were able to identify many so far missing proteins (PE level 2-4), and established together with our previously published studies on human erythrocytes (Lange et al. 2014) and platelets (Prudova et al. 2014), a general workflow suitable for the in-depth and high-throughput analysis of protein N-termini in human specimen, as envisioned by the Chromosome-centric Human Proteome Project.
HostingRepositoryPRIDE
AnnounceDate2015-08-18
AnnouncementXMLSubmission_2015-08-18_02:50:10.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUlrich ECKHARD
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList2-pyrrolidone-5-carboxylic acid (Glu); N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue; (R)-5-oxo-1: 4-tetrahydrothiazine-3-carboxylic acid; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); alpha-amino dimethylated residue
Instrument6520 Quadrupole Time-of-Flight LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-05-29 09:19:53ID requested
12015-08-18 02:50:11announced
Publication List
Eckhard U, Marino G, Abbey SR, Tharmarajah G, Matthew I, Overall CM, The Human Dental Pulp Proteome and N-Terminome: Levering the Unexplored Potential of Semitryptic Peptides Enriched by TAILS to Identify Missing Proteins in the Human Proteome Project in Underexplored Tissues. J Proteome Res, 14(9):3568-82(2015) [pubmed]
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
curator keyword: Biomedical
submitter keyword: Human, Dental Pulp, LC-MS/MS, TAILS N-terminomics, Agilent G6550A Q-TOF
Contact List
Prof. Christopher Mark Overall
contact affiliationCentre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada
contact emailchris.overall@ubc.ca
lab head
Ulrich ECKHARD
contact affiliationUniversity of British Columbia
contact emailulrich.eckhard@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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