PXD002264

PXD002264 is an original dataset announced via ProteomeXchange.

Title	Human Dental Pulp TAILS N-terminomics
Description	We report the targeted analysis of the human dental pulp proteome and N-terminome using the positional proteomics technique TAILS, which allowed us to capture both naturally blocked and unblocked protein N-termini. Furthermore, by using a proteomically barely studied human tissue in combination with a non-shotgun proteomics approach, we were able to identify many so far missing proteins (PE level 2-4), and established together with our previously published studies on human erythrocytes (Lange et al. 2014) and platelets (Prudova et al. 2014), a general workflow suitable for the in-depth and high-throughput analysis of protein N-termini in human specimen, as envisioned by the Chromosome-centric Human Proteome Project.
HostingRepository	PRIDE
AnnounceDate	2015-08-18
AnnouncementXML	Submission_2015-08-18_02:50:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich ECKHARD
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	2-pyrrolidone-5-carboxylic acid (Glu); N6: N6-dimethyl-L-lysine; iodoacetamide derivatized residue; (R)-5-oxo-1: 4-tetrahydrothiazine-3-carboxylic acid; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); alpha-amino dimethylated residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-29 09:19:53	ID requested
⏵ 1	2015-08-18 02:50:11	announced

Eckhard U, Marino G, Abbey SR, Tharmarajah G, Matthew I, Overall CM, The Human Dental Pulp Proteome and N-Terminome: Levering the Unexplored Potential of Semitryptic Peptides Enriched by TAILS to Identify Missing Proteins in the Human Proteome Project in Underexplored Tissues. J Proteome Res, 14(9):3568-82(2015) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biomedical

submitter keyword: Human, Dental Pulp, LC-MS/MS, TAILS N-terminomics, Agilent G6550A Q-TOF

Prof. Christopher Mark Overall
contact affiliation	Centre for Blood Research, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, Canada
contact email	chris.overall@ubc.ca
lab head
Ulrich ECKHARD
contact affiliation	University of British Columbia
contact email	ulrich.eckhard@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/08/PXD002264

PRIDE project URI

• PRIDE
  1. PXD002264
     1. Label: PRIDE project
     2. Name: Human Dental Pulp TAILS N-terminomics