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PXD002257

PXD002257 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of proteins interacting with extracellularly applied α-syn assemblies, Condition 1 - biological replicates (2/8)
DescriptionFibrillar α-Synuclein (α-Syn) is the principal component of Lewy bodies which are evident in individuals affected by Parkinson disease (PD). This neuropathologic form of α-Syn contributes to PD progression and propagation of exogeneous α-Syn between neurons has been demonstrated. In order to identify proteins interacting with extracellularly applied α-syn assemblies (either oligomeric or fibrillar α -syn) and identify in particular plasma membrane proteins exposed extracellularly, we exposed pure-neuronal cultures to oligomeric and fibrillar α-syn for 10 min, pulled down the complex, and identified the associated proteins using a proteomic-based approach. Using pull-down of whole cell lysates and MS, we have identified proteins interacting with extracellularly applied α-syn in three different conditions. Each condition consisted in three experimental replicates of cells exposed 10 min to α-syn assemblies and the non-treated cells used as controls.
HostingRepositoryPRIDE
AnnounceDate2015-09-07
AnnouncementXMLSubmission_2015-09-07_08:43:27.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD002257
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVirginie Redeker
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListOxidation; Acetyl; Carbamidomethyl
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02015-05-29 09:15:35ID requested
12015-09-07 08:43:28announced
Publication List
Shrivastava AN, Redeker V, Fritz N, Pieri L, Almeida LG, Spolidoro M, Liebmann T, Bousset L, Renner M, L, é, na C, Aperia A, Melki R, Triller A, 3-Na+/K+-ATPase and impair Na+ gradient. EMBO J, 34(19):2408-23(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: Alpha-synuclein, fibrils, oligomers, Protein-protein interactions, Pull-down, LC-MSMS, Proteomics
Contact List
Virginie Redeker; Ronald Melki
contact affiliationParis-Saclay Institute of Neuroscience, Team Melki, CNRS, Gif-sur-Yvette, France
contact emailVirginie.redeker@lebs.cnrs-gif.fr
lab head
Virginie Redeker
contact affiliationCNRS
contact emailvirginie.redeker@lebs.cnrs-gif.fr
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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