PXD002247

PXD002247 is an original dataset announced via ProteomeXchange.

Title	The proteome of native adult Müller cells from the murine retina
Description	We set out to first establish and validate a novel rapid method of enriching adult Müller cells, which yields sufficient cell numbers for proteomic profiling while keeping cells viable and morphologically, as well as physiologically fully intact. From these preparations we collected a comprehensive data set of the proteome of native murine Müller cells. Since many Müller cell functions depend on cell-cell interactions occurring at the plasma membrane level, we additionally included a subcellular fractionation approach that enabled to discriminate proteins primarily residing in the cytosol or the nucleus from those allocated in the plasma membrane and thus generate a first time Müller cell surfaceome study. We consider these data a first important step to better understanding the complex crosstalk between various retinal cell types and Müller cells as key players in health and ultimately also under disease conditions.
HostingRepository	PRIDE
AnnounceDate	2016-08-15
AnnouncementXML	Submission_2016-08-15_03:23:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002247
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Christine von Toerne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-28 07:43:33	ID requested
⏵ 1	2016-08-15 03:23:46	announced

Grosche A, Hauser A, Lepper MF, Mayo R, von Toerne C, Merl-Pham J, Hauck SM, ller Glial Cells From Murine Retina. Mol Cell Proteomics, 15(2):462-80(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: retina, LC-MSMS, MACS, FASP, Muller Glia cell

Stefanie Hauck
contact affiliation	Research Unit Protein Science Helmholtz Zentrum Muenchen German Research Center for Environmental Health (GmbH) D-85764 Neuherberg Germany Ph.: +49-89-3187-3941 Fax: +49-89-3187-4426
contact email	Hauck@helmholtz-muenchen.de
lab head
Christine von Toerne
contact affiliation	Helmholtz Zentrum München
contact email	vontoerne@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD002247

PRIDE project URI

• PRIDE
  1. PXD002247
     1. Label: PRIDE project
     2. Name: The proteome of native adult Müller cells from the murine retina