PXD002247 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The proteome of native adult Müller cells from the murine retina |
Description | We set out to first establish and validate a novel rapid method of enriching adult Müller cells, which yields sufficient cell numbers for proteomic profiling while keeping cells viable and morphologically, as well as physiologically fully intact. From these preparations we collected a comprehensive data set of the proteome of native murine Müller cells. Since many Müller cell functions depend on cell-cell interactions occurring at the plasma membrane level, we additionally included a subcellular fractionation approach that enabled to discriminate proteins primarily residing in the cytosol or the nucleus from those allocated in the plasma membrane and thus generate a first time Müller cell surfaceome study. We consider these data a first important step to better understanding the complex crosstalk between various retinal cell types and Müller cells as key players in health and ultimately also under disease conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2016-08-15 |
AnnouncementXML | Submission_2016-08-15_03:23:44.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD002247 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Christine von Toerne |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | Deamidated; Oxidation; Carbamidomethyl |
Instrument | LTQ Orbitrap XL |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2015-05-28 07:43:33 | ID requested | |
⏵ 1 | 2016-08-15 03:23:46 | announced | |
Publication List
Grosche A, Hauser A, Lepper MF, Mayo R, von Toerne C, Merl-Pham J, Hauck SM, ller Glial Cells From Murine Retina. Mol Cell Proteomics, 15(2):462-80(2016) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: retina, LC-MSMS, MACS, FASP, Muller Glia cell |
Contact List
Stefanie Hauck |
contact affiliation | Research Unit Protein Science Helmholtz Zentrum Muenchen German Research Center for Environmental Health (GmbH) D-85764 Neuherberg Germany Ph.: +49-89-3187-3941 Fax: +49-89-3187-4426 |
contact email | Hauck@helmholtz-muenchen.de |
lab head | |
Christine von Toerne |
contact affiliation | Helmholtz Zentrum München |
contact email | vontoerne@helmholtz-muenchen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/08/PXD002247 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD002247
- Label: PRIDE project
- Name: The proteome of native adult Müller cells from the murine retina