PXD002243

PXD002243 is an original dataset announced via ProteomeXchange.

Title	The effect of oxygen on bile resistance in L. monocytogenes
Description	Listeria monocytogenes is a gram-positive, facultative anaerobe food-borne pathogen that is the causative agent of listeriosis. Upon ingestion, L. monocytogenes is subjected to a variety of non-specific host defenses such as bile. The main component of bile is bile salts which is known to be bactericidal through inducing DNA damage, oxidative damage, membrane instability, and protein misfolding. Previous studies have focused mainly on aerobic conditions; however the human GI tract is an environment ranging from microaerophilic to anaerobic. The bile salt hydrolase, an enzyme known to reduce toxicity of bile through hydrolysis, has been shown to have an increase in activity under anaerobic conditions. Therefore, the purpose of this study was to determine the effect of oxygen on bile resistance and determine the bile specific proteome that is responsible for this. To do this, we performed survival assays on virulent strains: F2365, 10403S, EGDe and avirulent strain, HCC23. Results showed an increase in viability for all virulent strains when exposed to porcine bile under anaerobic conditions. Interestingly, an increase in resistance was seen in HCC23 only under aerobic conditions. We then used a total proteomic analysis approach to compare the bile specific proteome under both aerobic and anaerobic conditions. Results showed many difference between all strains including an increase in abundance of proteins associated with stress responses, repair, cell morphology, and cell division under anaerobic conditions. Response to bile salt stress showed to be strain dependent. This study not only identified proteins responsible for L. monocytogenes bile resistance but also differerences between aerobic and anaerobic conditions thus suggesting that oxygen availability is not only a stressor but in some way providing assistance to overcoming bile salts.
HostingRepository	PRIDE
AnnounceDate	2017-05-03
AnnouncementXML	Submission_2017-05-03_08:28:43.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD002243
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Joseph Reddy
SpeciesList	scientific name: Listeria monocytogenes; NCBI TaxID: 1639;
ModificationList	dihydroxylated residue; amidated residue; dehydrated residue; phosphorylated residue; acetylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	LTQ Velos

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-28 02:45:03	ID requested
⏵ 1	2017-05-03 08:28:46	announced

Wright ML, Pendarvis K, Nanduri B, Edelmann MJ, Jenkins HN, Reddy JS, Wilson JG, Ding X, Broadway PR, Ammari MG, Paul O, Roberts B, Donaldson JR, . J Proteomics Bioinform, 9(4):107-119(2016) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Listeria monocytogenes, 10403S, F2365, HCC23, EGD, EGDe

Janet Donaldson
contact affiliation	Biological Sciences Dept, Mississippi State University, Mississippi State, 39762
contact email	donaldson@biology.msstate.edu
lab head
Joseph Reddy
contact affiliation	Basic Sciences, College of Veterinary Medicine
contact email	jsr256@msstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD002243
     1. Label: PRIDE project
     2. Name: The effect of oxygen on bile resistance in L. monocytogenes