PXD002241

PXD002241 is an original dataset announced via ProteomeXchange.

Title	USSC secretome
Description	Stem cell transplantation is a promising therapeutic strategy to enhance axonal regeneration after spinal cord injury. Unrestricted somatic stem cells (USSC) isolated from human umbilical cord blood is an attractive stem cell population available at GMP grade without any ethical concerns. It has been shown that USSC transplantation into acute injured rat spinal cords leads to axonal regrowth and significant locomotor recovery, yet lacking cell replacement. Instead, USSC secrete trophic factors enhancing neurite growth of primary cortical neurons in vitro. Here, we applied a functional secretome approach characterizing proteins secreted by USSC for the first time and validate candidate neurite growth promoting factors using primary cortical neurons in vitro. By mass spectrometric analysis and exhaustive bioinformatic interrogation we identified 1156 proteins representing the secretome of USSC. Using Gene Ontology we revealed that USSC secretome contains proteins involved in a number of relevant biological processes of nerve regeneration such as cell adhesion, cell motion, blood vessel formation, cytoskeleton organization and extracellular matrix organization. We found for instance that 31 well-known neurite growth promoting factors like, e.g., neuronal growth regulator 1, NDNF, SPARC and PEDF spanning the whole abundance range of USSC secretome. By the means of primary cortical neurons in vitro assays we verified SPARC and PEDF as significantly involved in USSC mediated neurite growth and therewith underline their role in improved locomotor recovery after transplantation. From our data we are convinced that USSC are a valuable tool in regenerative medicine as USSC’s secretome contains a comprehensive network of trophic factors supporting nerve regeneration not only by a single process but also maintained its regenerative phenotype by a multitude of relevant biological processes.
HostingRepository	PRIDE
AnnounceDate	2015-07-20
AnnouncementXML	Submission_2015-07-27_04:59:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jessica Schira
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2015-05-28 01:29:07	ID requested
1	2015-07-20 06:55:01	announced
⏵ 2	2015-07-27 04:59:49	announced	Updated publication reference for PubMed record(s): 26183719.

Schira J, Falkenberg H, Hendricks M, Waldera-Lupa DM, K, ö, gler G, Meyer HE, M, ü, ller HW, St, ü, hler K, Characterization of Regenerative Phenotype of Unrestricted Somatic Stem Cells (USSC) from Human Umbilical Cord Blood (hUCB) by Functional Secretome Analysis. Mol Cell Proteomics, 14(10):2630-43(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: secretome, USSC, stem cell, spinal cord, nerve regeneration, absolute quantification

Jessica Schira
contact affiliation	MPL-BMFZ Heinrich Heine Universität Düsseldorf
contact email	j.schira@uni-duesseldorf.de
lab head
Jessica Schira
contact affiliation	MPL-BMFZ
contact email	j.schira@uni-duesseldorf.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/07/PXD002241

PRIDE project URI

• PRIDE
  1. PXD002241
     1. Label: PRIDE project
     2. Name: USSC secretome